基于肝微粒体和指纹图谱研究六味地黄丸与硝苯地平的药物相互作用  

Investigation of herb-drug interaction between Liuwei Dihuang Pills and nifedipine based on rat liver microsomes and HPLC fingerprints

作　　者：郑梅 刘富垒[2] 薛敬伟[2] 柳文媛[1] ZHENG Mei;LIU Fulei;XUE Jingwei;LIU Wenyuan(Key Laboratory of Drug Quality Control and Pharmacovigilance(China Pharmaceutical University),Ministry of Education,Nanjing 210009;Taian City Central Hospital,Shandong 271000,China)

机构地区：[1]药物质量与安全预警教育部重点实验室(中国药科大学),南京210009 [2]山东省泰安市中心医院,泰安271000

出　　处：《中国药科大学学报》2018年第2期195-201,共7页Journal of China Pharmaceutical University

基　　金：山东省自然科学基金资助项目(No.ZR2014HL108);山东省优秀中青年科学家科研奖励基金资助项目(No.ZR2015HL130)~~

摘　　要：本研究建立了基于体外肝微粒体和中药代谢指纹图谱研究六味地黄丸与硝苯地平的药物相互作用的方法。体外肝微粒体温孵采用加入六味地黄丸和硝苯地平置37℃水浴中温孵60 min。指纹图谱测定采用C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%甲酸为流动相梯度洗脱,流速1.0 m L/min,检测波长240 nm;测定温孵前后微粒体中六味地黄丸的代谢指纹图谱,通过相似度比较评价两者的相互作用。结果显示六味地黄丸与硝苯地平共孵前后,代谢指纹图谱无明显差异。在体动物试验显示,六味地黄丸与硝苯地平联合给药前后,典型成分的主要药代动力学参数无显著差异。A new method based on rat liver microsomes and chromatographic fingerprint comparison was established to investigate the possible herb-drug interactions between Liuwei Dihuang Pills(LDP)and nifedipine(NIF).LDP and NIF were incubated simultaneously with rat liver microsomes at 37°C for 60 min in a shaking water bath.The separation was achieved on a C 18 column using 0.1%formic acid solution and acetonitrile as mobile phase with a liner gradient program.The flow rate was set at 1.0 mL/min.Detection was achieved by UV light at 240 nm.To evaluate the interactions between LDP and NIF,the similarity of the fingerprinting chromatograms before and after co-incubation was calculated by similarity evaluation system for chromatographic fingerprint of TCM.Furthermore,pharmacokinetic experiments in rat suggested that there was no significant difference in the pharmacokinetic parameters of the typical components in LDP.

关 键 词：六味地黄丸 硝苯地平 肝微粒体 指纹图谱 药物相互作用 药代动力学 

分 类 号：R917[医药卫生—药物分析学]