过氧化物酶V基因沉默HepG2肝癌细胞系的构建及鉴定  

Establishment and Identification of Peroxiredoxin V Silencing HepG2 liver Cancer Cell Line

作　　者：宫伊希 刘悦[1] 王闯[1] 焦冰洋 韩英浩[1] 金成浩[1] 孙虎男[1] Gong Yixi;Liu Yue;Wang Chuang;Jiao Bingyang;Han Yinghao;Jin Chenghao;Sun Hunan(College of Life Science and Technology,HeilongjiangBayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2018年第2期52-55,99,共5页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学科研培育基金(XA2014-04)

摘　　要：过氧化物酶V(Peroxiredoxin V,Prx V)是过氧化物酶家族(Peroxiredoxins)中的一员,具有清除细胞内活性氧(Reactive Oxygen Species,ROS)从而抑制由于氧化应激导致的细胞凋亡的作用。该研究通过慢病毒载体构建Prx V基因沉默的HepG2肝癌稳定细胞系。采用荧光照相及蛋白免疫印迹法对其结果进行鉴定,为研究Prx V的功能提供良好的基础。结果表明:成功构建Prx V基因沉默的HepG2肝癌细胞系。Peroxiredoxin V(Prx V)was a thioredoxin peroxidase involved in peroxiredoxins family and it was also known as reactive oxygen species(ROS)scavenger which played a role in protecting the oxidative stress induced cell apoptosis.The Prx V knockdown HepG2 cell line was established throughlentivirus vectors and was analyzed the protein levels by western blot.The results showed that throughthe lentivirus vector treatments the Prx V knockdown HepG2 cell line was successfully established.

关 键 词：慢病毒载体 过氧化物酶V HepG2肝癌细胞系 

分 类 号：TS22[轻工技术与工程—粮食、油脂及植物蛋白工程]