脱痂软膏中氧化锌和龙脑的含量测定  

Contents determination of zinc oxide and borneol in Tuojia ointment

作　　者：王晓君[1] 徐晓阳[2] 陆松伟[1] 虞慧华[1] 宋洪杰[1] WANG XiaoJun;XU XiaoYang;LU SongWei;YU HuiHua;SONG HongJie(Department of Pharmacy,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;Teaching Office of Pharmaceutical Analysis,School of Pharmacy,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]第二军医大学长海医院药学部,上海200433 [2]郑州大学药学院药物分析学教研室,郑州450001

出　　处：《药学服务与研究》2018年第2期117-119,126,133,共4页Pharmaceutical Care and Research

基　　金：上海市科学技术委员会科研计划项目(13401902300);军队医疗机构制剂标准提高科研专项课题(13ZJZ11-3)

摘　　要：目的:建立脱痂软膏中氧化锌和龙脑的含量测定方法。方法:采用配位滴定法测定氧化锌含量,通过考察水浴温度和时间、稀盐酸和磷酸氢二钠试液用量优化测定条件。采用气相色谱法测定龙脑含量。气相色谱条件:DB-5ms毛细管色谱柱(30m×0.25mm,0.25μm);氢火焰离子化检测器(FID);载气:氦气,流速1.0ml/min;进样口温度:180℃;柱温:85℃;检测器温度:200℃;分流比为10∶1。结果:氧化锌的平均回收率为(94.77±0.14)%(n=6)。龙脑在0.053 7~0.214 8mg/ml范围内线性关系良好(r=0.999 5),平均加样回收率为(96.34±0.74)%(n=6)。结论:所建立的方法能够快速、准确地定量检测处方中的氧化锌和龙脑,可用于脱痂软膏的质量控制。Objective:To establish methods for content determination of zinc oxide and borneol in Tuojia ointment.Methods:Coordination titration was used for content determination of zinc oxide.The determination conditions were optimized through investigating the time and temperature of water bath,the amount of diluted hydrochloric acid and disodium hydrogen phosphate solution.The content of borneol was determined by gas chromatography.Separation was carried out on a DB-5ms capillary column(30 m X0.25 mm,0.25^m)with He as a carrier gas at a flow rate of 1.0 ml/min.The column temperature was 85 C.The temperatures of injector and flame ionization detector were 180 C and 200 C,respectively.The injection split ratio was 10:1.Results:The average recovery of zinc oxide was(94.77+0.14)%(n-6).Borneol displayed good linearity within the range of 0.053 7-0.214 8 mg/ml(r=0.999 5)and average recovery was(96.34士0.74)%(n=6).Conclusion:The established methods could determine the contents of zinc oxide and borneol in Tuojia ointment rapidly,reliably and quantitative-ly,and could be used for quality control of Tuojia ointment.

关 键 词：脱痂软膏 氧化锌 龙脑 含量测定 色谱法 气相 配位滴定 

分 类 号：R927.2[医药卫生—药学]