我国部分地区鸭1型甲肝病毒流行株遗传变异分析  

Epidemiology and Genetic Variation of Duck Hepatitis A Virus Type 1 Circulating in Some Regions of China

作　　者：傅光华[1,2] 陈翠腾 温名根[4] 施少华 陈红梅 万春和[1,2] 傅秋玲 程龙飞[1,2] 刘荣昌 黄瑜[1,2,3] FU Guang-hua;CHEN Cui-teng;WEN Ming-gen;SHI Shao-hua;CHEN Hong-mei;WAN Chun-he;FU Qiu-ling;CHENG Long-fei;LIU Rong-chang;HUANG Yu(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention,Fuzhou,Fujian 350013,China;Fujian Animal Diseases Control Technology Center,Fuzhou,Fujian 350013,China;Bureau of Animal Husbandry and Veterinary Medicine of Ji′an County of Jiangxi,Ji′an,Jiangxi 343100,China)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013 [2]福建省禽病防治重点实验室,福建福州350013 [3]福建省畜禽疫病防治工程技术研究中心,福建福州350013 [4]江西省吉安县畜牧兽医局,江西吉安343100

出　　处：《福建农业学报》2018年第2期109-113,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2015J01113);福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1023-3);现代农业(水禽)产业技术体系建设专项(CARS-42);福建省农业科学院青年英才计划项目(YC2015-13)

摘　　要：为了解鸭群中鸭1型甲肝病毒(DHAV-1)的流行及其分子变异情况,对2015-2016年福建、广东、浙江及江西鸭群采集的327份临床样品进行检测、病毒分离鉴定、序列测定与分析。结果表明,自327份临床样品中检出DHAV-1阳性样品35份,阳性率10.7%;从阳性样品中分离获得23株DHAV-1,主要来自30日龄以内的麻鸭和半番鸭。对DHAV-1的VP1基因分子特征及遗传变异分析,表明23株病毒VP1基因核苷酸序列同源性介于93.3%~99.9%,分属2个病毒亚群,两亚群间的遗传距离为0.06。23株临床分离株中有18株与引起雏鸭胰腺泛黄的毒株(MPZJ1206株)处于同一亚群,为目前流行的优势毒株,与国内外疫苗株核苷酸同源性在91.3%~96.2%,存在较大差异。由此可见,我国福建省及周边地区鸭群中不同DHAV-1流行株之间及流行毒株与疫苗株间均存在不同程度的差异。The epidemic prevalence and molecular variation of duck hepatitis A virus type 1(DHAV-1)were investigated.Three-hundred-twenty-seven clinical samples collected in 2015-2016 from Fujian and surrounding provinces were used for the virus detection and isolation.Thirty-five of these samples(10.7%)were found to be pusitive for DHAV-1.From the 35 positive specimens,23 strains were isolated,mainly from shelducks and mule ducks of less than 30-day-old.The sequence analysis on VP1 gene of the 23 isolates showed 93.3%-99.9%homology,and the strains could be divided into 2 subgroups with a genetic distance of 0.06.Among them,18 isolates clustered in a currently prevalent subgroup which contains the virus(MPZJ1206)causing yellowed-pancreas in ducklings.On the other hand,all 23 isolates shared merely 91.3-96.2%nucleotide sequence homology with the strain used for vaccination abroad and domestically.It appeared that genetic variations existed among the various DHAV-1 strains.

关 键 词：鸭1型甲肝病毒 VP1基因 变异分析 

分 类 号：S852[农业科学—基础兽医学]