白及种子非共生培养高效萌发体系的研究  

Efficient Germination System in Asymbiotic Culture of Bletilla Striata(Thunb.) Reichb. F.

作　　者：陈菁瑛[1,2] 万学锋[2,3] 刘保财 赵云青[1,2] 黄颖桢[1,2] 张武君[1,2] CHEN Jing-ying;WAN Xue-feng;LIU Bao-cai;ZHAO Yun-qing;HUANG Ying-zhen;ZHANG Wu-jun(Institute of Agricultural Bioresource,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Research Center for Medicinal Plant,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Zhangzhou Institute of Agricultural sciences,Zhangzhou,Fujian 363005,China)

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]福建省农业科学院药用植物研究中心,福建福州350003 [3]福建省漳州市农业科学研究所,福建漳州363005

出　　处：《福建农业学报》2018年第2期131-135,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省农业科学院"十三五"科技创新团队--药用植物创新团队项目(2017);福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1018-1;2016R1017-1)

摘　　要：以白及近成熟蒴果为材料,无菌播种于MS基本培养基上进行非共生培养研究,比较了播种法、播种量、贮藏法、光照及添加物等对种子萌发的影响,建立白及种子非共生培养高效萌发体系。结果表明:白及种子适宜采用5×10~3粒·mL^(-1)密度悬液法播种于MS培养基上,并在播种后前14d进行黑暗培养,萌发率达93%。添加10%土豆泥或椰汁能有效提高萌发率,促进原球茎的生长。塑料袋包装低温贮藏利于白及种子保存,贮藏60d仍有一半萌发。The mature capsule of Bletilla striata was used as material for asymbiotic germination in this experiment.With MS as basal medium,effects of different sowing ways,seedling rates,storage ways,light conditions and additives on the seed germination of Bletilla striata were studied.The results showed that the best germination conditions for asymbiotic culture of Bletilla striata is with seedling rate 5×10 3 grains·mL-1 in suspended liquid,and cultured in dark conditions for the first 14 days,the germination rate of which reached 93%.Adding 10%mashed potato or coconut juice could increase the germination rate,and promote the growth of protocorm.The seeds of Bletilla striata stored in plastics bags at 4℃was beneficial for germination,the germination rate for which could reach 50%after 60 days.

关 键 词：白芨 种子 非共生培养 萌发 

分 类 号：S567.239[农业科学—中草药栽培]