不同比色法测定植物源总三萜皂苷含量的对比  

Comparation of Different Colorimetry for Determination of the Total Herbal Triterpenoid Saponins

作　　者：陈钰泉 刘玉婷 邱杰 吴东美 谢文佩 谭强 CHEN Yu-quan;LIU Yu-ting;QIU Jie;WU Dong-mei;XIE Wen-pei;TAN Qiang(Institute of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医大学药学院,广西南宁530001

出　　处：《黑龙江农业科学》2018年第3期108-112,共5页Heilongjiang Agricultural Sciences

基　　金：广西自然科学基金资助项目(2014GX NSFAA118176;2012GXNSFAA276037);南宁市科技计划资助项目(KJT13088);广西中医大学硕士研究生科研创新资助项目(YJS201608)

摘　　要：为建立多种植物混合提取液总三萜皂苷含量的测定方法,通过比较无患子、艾草、皂荚及茶籽粕4种植物源三萜皂苷与人参皂苷Rh_2在4种显色体系(高氯酸、浓硫酸-甲醇、香草醛乙醇-浓硫酸、香草醛冰醋酸-高氯酸-冰醋酸)中最大吸收波长的差异,选择差异最小的体系作为最佳显色体系测定这4种植物源总三萜皂苷。结果表明:高氯酸作为显色体系测得的4种植物源三萜皂苷与人参皂苷最大吸收波长的差异最小,作为最佳的检测系统。当无患子、艾草、皂荚及茶籽粕以重量比为2.0∶2.0∶1.0∶1.5,测得混合提取液总三萜皂苷含量为(166.2012±2.2615)mg·g^(-1)。说明高氯酸法简便、可靠且重复性好,可作为无患子、艾草、皂荚及茶籽粕等混合提取液总三萜皂苷含量的测定方法。In order to establish the colorimetric method for determination of total triterpenoid saponins from four herbs including Sapindus mukurossi,argy wormwood leaf,Gleditsia sinensis and tea seed meal.By analyzing the maximum absorption wavelength of the herbal extracts and ginsenoside Rh 2 in four chromogenic systems included perchloric acid,concentrated sulfuric acid-methanol,vanillin ethanol-sulfuric acid,vanillin-acetic acid-perchloric acid-acetic acid,the optimal system for the determination of total saponins was obtained.The results showed that the best chromogenic system was perchloric acid due to the minimum difference between the maximum absorption wavelength of herbal saponins and that of ginsenoside Rh 2.The total triterpenoid saponins from the mixed extracts from the four herbs with the mass ratio(w/w)of 2.0∶2.0∶1.0∶1.5 were(166.201 2±2.261 5)mg·g-1.The colorimetric method for determination of total herbal triterpenoid saponins was convenient,reliable and reproducible,and can be used as the quality control of total saponins from different herbs.

关 键 词：比色法 三萜皂苷 高氯酸 最大吸收波长 

分 类 号：Q946[生物学—植物学]