雷诺嗪预处理对大鼠心肌缺血再灌注损伤保护作用的研究  

Protective effects of ranolazine preconditioning on myocardial ischemia reperfusion injury in rats

作　　者：赵可欣[1] 刘明远 李萌 杨军[1] 王立波[1] 董天崴[1] 孟祥冬[1] 杨光远[1] ZHAO Ke-xin;LIU Ming-yua;LI Meng;YANG Jun;WANG Li-bo;DONG Tian-wei;MENG Xiang-dong;YANG Guang-yuan(Depertment of Cardiology,First Affiliated Hospital of Jiamusi University,Jiamusi,Heilongjiang,154002,China)

机构地区：[1]佳木斯大学附属第一医院心内科,黑龙江佳木斯154002 [2]佳木斯大学基础医学院药理学教研室

出　　处：《心血管康复医学杂志》2018年第2期149-154,共6页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：2016年佳木斯大学研究生科技创新项目(YM2016_062);黑龙江省科学基金项目(LC2016039)~~

摘　　要：目的:研究雷诺嗪预处理对大鼠心肌缺血再灌注损伤(MIRI)的保护作用。方法:将32只SD大鼠随机均分为假手术组、缺血再灌注(I/R)组、低剂量雷诺嗪组(低剂量组)、高剂量雷诺嗪组(高剂量组),测量比较各组HR、SBP、DBP、左室收缩压(LVSP)、左室舒张压(LVDP)、左室内压最大上升速率(+dp/dt_(max))、左室内压最大下降速率(-dp/dt_(max))、CK-MB、LDH、cTnI水平、心肌梗死严重程度、三磷酸腺苷(ATP)含量的差异。结果:与假手术组比较,I/R组、低剂量组、高剂量组LVSP[(119.35±5.00)mmHg比(92.68±2.95)mmHg比(100.60±3.12)mmHg比(112.22±3.69)mmHg]、LVDP[(24.78±1.71)mmHg比(17.26±1.69)mmHg比(19.25±1.05)mmHg比(22.18±1.55)mmHg]、+dp/dt_(max)[(3736±102.37)mmHg/s比(3115±112.72)mmHg/s比(3338±51.88)mmHg/s比(3446±37.99)mmHg/s]、-dp/dt_(max)[(3634±102.51)mmHg/s比(3015±127.00)mmHg/s比(3239±37.36)mmHg/s比(3349±45.49)mmHg/s]和ATP含量[(22.54±1.52)nmol/mg比(14.08±1.80)nmol/mg比(16.88±0.74)nmol/mg比(19.34±0.88)nmol/mg]显著降低,CK-MB[(490.88±168.04)U/L比(1259.0±78.02)U/L比(1127.9±127.23)U/L比(956.62±105.22)U/L]、LDH[(1494.9±174.84)U/L比(2657.6±104.33)U/L比(2293.9±99.58)U/L比(1932.6±134.25)U/L]、cTnI[(1.03±0.14)ng/ml比(10.62±1.34)ng/ml比(6.97±1.32)ng/ml比(4.87±0.79)ng/ml]水平显著升高(P均<0.01)。与I/R组比较,低剂量组、高剂量组LVSP、LVDP、+dp/dt_(max)、-dp/dt_(max)和ATP含量显著升高,CK-MB、LDH、cTnI水平和心肌梗死程度[(0.5289±0.0223)比(0.4887±0.0089)比(0.4438±0.0154)]显著降低(P<0.05或<0.01),且高剂量组显著优于低剂量组(P<0.05或<0.01)。结论:雷诺嗪预处理对心肌缺血再灌注损伤有显著保护作用,且呈剂量依赖性。Objective:To study protective effects of ranolazine preconditioning on myocardial ischemia reperfusion injury(MIRI)in rats.Methods:A total of 32 SD rats were randomly and equally divided into sham operation group,ischemia/reperfusion(I/R)group,low dose ranolazine group(low dose group)and high dose ranolazine group(high dose group).HR,SBP,DBP,left ventricular systolic pressure(LVSP),left ventricular diastolic pressure(LVDP),left ventricular pressure maximum rate of rise(+dp/dt max),left ventricular pressure maximum rate of decline(-dp/dt max),levels of CK-MB,LDH and cTnI,severity of myocardial infarction and ATP concentration were measured and compared among all groups.Results:Compared with sham operation group,there were significant reductions in LVSP[(119.35±5.00)mmHg vs.(92.68±2.95)mmHg vs.(100.60±3.12)mmHg vs.(112.22±3.69)mmHg],LVDP[(24.78±1.71)mmHg vs.(17.26±1.69)mmHg vs.(19.25±1.05)mmHg vs.(22.18±1.55)mmHg],+dp/dt max[(3736±102.37)mmHg/s vs.(3115±112.72)mmHg/s vs.(3338±51.88)mmHg/s vs.(3446±37.99)mmHg/s],-dp/dt max[(3634±102.51)mmHg/s vs.(3015±127.00)mmHg/s vs.(3239±37.36)mmHg/s vs.(3349±45.49)mmHg/s]and ATP concentration[(22.54±1.52)nmol/mg vs.(14.08±1.80)nmol/mg vs.(16.88±0.74)nmol/mg vs.(19.34±0.88)nmol/mg],and significant rise in levels of CK-MB[(490.88±168.04)U/L vs.(1259.0±78.02)U/L vs.(1127.9±127.23)U/L vs.(956.62±105.22)U/L],LDH[(1494.9±174.84)U/L vs.(2657.6±104.33)U/L vs.(2293.9±99.58)U/L vs.(1932.6±134.25)U/L]and cTnI[(1.03±0.14)ng/ml vs.(10.62±1.34)ng/ml vs.(6.97±1.32)ng/ml vs.(4.87±0.79)ng/ml]in I/R group,low dose group and high dose group,P<0.01 all.Compared with I/R group,there were significant rise in LVSP,LVDP,+dp/dt max,-dp/dt max and ATP concentration,and significant reductions in levels of CK-MB,LDH and cTnI and MI severity[(0.5289±0.0223)vs.(0.4887±0.0089)vs.(0.4438±0.0154)]in low dose group and high dose group(P<0.05 or<0.01),and those of high dose group were significantly better than those of low dose group(P<0.05 or<0.01).Conclusion:Ranolazine precond

关 键 词：心肌缺血 再灌注损伤 雷诺嗪 

分 类 号：R542.2[医药卫生—心血管疾病]