芪茵颗粒对非酒精性脂肪性肝病大鼠肝细胞胰岛素受体底物1及磷脂酰肌醇3激酶的影响研究  

Study on the effects of Qi Yin Granule on PI-3K and IRS-1 in rats with nonalcoholic fatty liver disease

作　　者：王媛媛 蒙婷 赵晓咏 曾斌芳[3] Wang Yuanyuan;Meng Ting;Zhao Xiaoyong;ZENG Binfang(Department of Traditional Chinese Medicine,Mingyuan Staff Hospital of Xinjiang Oilfield Company,Xinjiang,Urumqi 830000)

机构地区：[1]新疆油田公司明园职工医院中医科,新疆乌鲁木齐830000 [2]新疆油田公司明园职工医院综合内科,新疆乌鲁木齐830000 [3]新疆医科大学中医学院,新疆乌鲁木齐830011

出　　处：《河北中医》2018年第4期569-574,588,共7页Hebei Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81460705)

摘　　要：目的观察芪茵颗粒对非酒精性脂肪性肝病(NAFLD)大鼠肝细胞胰岛素受体底物1(IRS-1)及磷脂酰肌醇3激酶(PI-3K)m RNA表达的影响,从胰岛素抵抗(IR)途径来阐述芪茵颗粒对NAFLD的治疗作用。方法将72只SD雄性大鼠随机分为6组,即空白组、模型组、硫普罗宁组、芪茵颗粒高剂量组、芪茵颗粒中剂量组及芪茵颗粒低剂量组,每组各12只。空白组大鼠常规饲料喂养,剩余5组行高脂乳剂灌胃至实验第10周后,空白组及模型组大鼠予0.9%氯化钠注射液10 m L/(kg·d)灌胃,硫普罗宁组予硫普罗宁20 mg/(kg·d)灌胃,芪茵颗粒高、中、低剂量组分别予芪茵颗粒水煎剂10、5、2.5 g生药/(kg·d)灌胃,连续灌胃至第20周结束。观察比较6组大鼠甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及丙氨酸氨基转移酶(ALT)水平,计算肝指数,光学显微镜下观察比较肝组织病理形态及非酒精性脂肪性肝病活动度评分(NAS),实时荧光定量聚合酶链式反应(RT-PCR)法测定大鼠肝组织IRS-1、PI-3K m RNA的表达。结果与模型组相比,硫普罗宁组及芪茵颗粒低、中、高剂量组TC、TG、LDL-C及ALT水平降低(P<0.05),HDL-C水平升高(P<0.05);与硫普罗宁组及芪茵颗粒低剂量组相比,芪茵颗粒高剂量组TC、TG、LDL-C及ALT水平更低(P<0.05),HDL-C水平更高(P<0.05)。与模型组相比,芪茵颗粒中、高剂量组大鼠体质量均下降(P<0.05),硫普罗宁组及芪茵颗粒低、中、高剂量组肝湿质量及肝指数均降低(P<0.05)。与模型组相比,硫普罗宁组及芪茵颗粒中、高剂量组NAS均降低(P<0.05);与硫普罗宁组相比,芪茵颗粒中剂量组NAS降低(P<0.05);与芪茵颗粒低剂量组相比,芪茵颗粒中、高剂量组NAS降低(P<0.05)。与模型组相比,硫普罗宁组及芪茵颗粒中、高剂量组IRS-1、PI-3K m RNA表达均明显升高(P<0.05);与硫普罗宁组及芪茵颗粒低剂量组相比,芪茵颗粒中�Objective To observe the effect of Qi yin granule on the expression of insulin receptor substrate 1(IRS-1)and phosphatidylinositol 3 kinase(PI-3K)mRNA in the liver cells of nonalcoholic fatty liver disease(NAFLD)rats.The effect of Qi yin granule on NAFLD was discussed from the insulin resistance(IR)pathway.Methods 72 SD male rats were randomly divided into six groups:blank group,model group,tiopronin group,low dose,middle and high dose group of Qi yin granule,12 rats in each group.Rats in blank group were routinely reared,and after the remaining 5 groups were given high-fat emulsion by gavage for 10 weeks,rats in blank group and model group were given daily 0.9%sodium chloride injection 10 mL/(kg·d)by gavage,and tiopronin group was given tiopronin 20 mg/(kg·d)by gavage every day.The high,middle,and low doses of Qi yin granule were given to 10,5,and 2.5 g/(kg·d)water extracts respectively,and they were continuously administered to the end of the 20th week.The levels of serum triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)and alanine aminotransferase(ALT)in six groups were observed and compared.The liver index was calculated and the pathological changes of liver tissue were observed through light microscope and nonalcoholic fatty liver disease activity score(NAS)were compared and the expression of IRS-1 and PI-3KmRNA in liver tissue of rats was measured by real-time fluorescence quantitative polymerase chain reaction(Real-time PCR).Results Compared with the model group,the levels of TC,TG,LDL-C and ALT in the tiopronin group and the low,middle,and low doses of the Qi yin granule were decreased(P<0.05),and the HDL-C level was increased(P<0.05).Compared with the tiopronin group and the low-dose Qi yin granule group,the TC,TG,LDL-C,ALT levels were lower in the highdose Qi yin granule group and the HDL-C level was lower(P<0.05).Compared with the model group,the body weight of the middle and high dose group of Qi yin granule were decreased(

关 键 词：脂肪肝 疾病模型 动物 中医疗法 1-磷脂酰肌醇3-激酶 受体 胰岛素 

分 类 号：R329.447[医药卫生—人体解剖和组织胚胎学] R285.5[医药卫生—基础医学]