失用性骨质疏松模型大鼠血清Ⅰ型胶原羧基端交联肽与骨密度的相关性  

Correlation of serum C-telopeptide cross-linked collagen type I with bone mineral density in a rat model of disuse osteoporosis

作　　者：易开君 王莲朋 丁慧勇[1] 孔丹[1] 韩亚军[1] 马风华[1] 段蔚楠 周高晋 李国华[1] Yi Kai-jun;Wang Lian-peng;Ding Hui-yong;Kong Dan;Han Ya-jun;Ma Feng-hua;Duan Wei-nan;Zhou Gao-jin;Li Guo-hua(Department of Orthopedics,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi830000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第二附属医院骨科,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究》2018年第12期1872-1876,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金(2015211C102)~~

摘　　要：背景:Ⅰ型胶原羧基端交联肽和Ⅰ型胶原氨基端前肽是目前敏感性较好的骨代谢标记物,能够反映骨形成和骨吸收的状态。目的:分析Ⅰ型胶原羧基端交联肽与失用性骨质疏松症的关系。方法:雄性SD大鼠,体质量180-220 g,随机分成对照组和失用性骨质疏松症组。大鼠采用踝尾固定法将右后肢固定4周,建立失用性骨质疏松症大鼠模型;其余大鼠为对照组,麻醉后不做任何处理。造模前后行腹腔静脉采血,再取出股骨,检测SD大鼠血清Ⅰ型胶原羧基端交联肽和两侧股骨骨密度值。结果与结论:(1)造模前,两组大鼠血清Ⅰ型胶原羧基端交联肽水平相近(P>0.05);造模4周后,失用性骨质疏松症组血清Ⅰ型胶原羧基端交联肽水平显著高于造模前和对照组(P<0.01);对照组2个时间点血清Ⅰ型胶原羧基端交联肽浓度比较差异无显著性意义(P>0.05);(2)失用性骨质疏松症组造模前后血清Ⅰ型胶原羧基端交联肽差值与两侧股骨骨密度差值呈正相关性(r=0.426,P<0.01);(3)失用性骨质疏松症组左股骨骨密度高于右股骨骨密度(P<0.01),对照组两侧股骨骨密度比较差异无显著性意义(P>0.05),失用性骨质疏松症组右股骨骨密度显著低于对照组(P<0.01);(4)结果说明,踝尾固定建立失用性骨质疏松症模型切实可行;失用性骨质疏松症可促进Ⅰ型胶原羧基端交联肽的生成,致骨密度减少,Ⅰ型胶原羧基端交联肽与骨量丢失程度呈正相关,可用于骨质疏松的疗效评价和诊断。BACKGROUND:C-telopeptide and N-telopeptide cross-linked collagen type I(CTx and NTx,respectively)are specific biochemical bone markers that can reflect bone formation and resorption.OBJECTIVE:To analyze the association of CTx with disuse osteoporosis.METHODS:Male Sprague-Dawley rats,weighing 180-220 g,were randomly divided into control and disuse osteoporosis groups.Right hind limbs of the rats in the disuse osteoporosis group were immobilitzed for 4 weeks by ankle-tail fixation to establish the rat model of disuse osteoporosis.Peritoneal venous blood was collected before and after modeling,and the femur was then removed to measure the serum CTx level and bone mineral density of the bilateral femurs.RESULTS AND CONCLUSION:The serum CTx level did not differ significantly between groups before modeling(P>0.05).At 4 weeks after modeling,the serum CTx level in the disuse osteoporosis group was significantly higher than that in the control group and at baseline(P<0.01).The serum CTx level showed no significant change in the control group before and after modeling(P>0.05).The increment of serum CTx in the disuse osteoporosis group exhibited a negative correlation with the bone mineral density of the bilateral femurs(r=0.426,P<0.01).The bone mineral density of the right femur in the disuse osteoporosis group was significantly lower than that of the left one in the disuse osteoporosis group and the right one in the control group(P<0.01),and there was no significant difference in the bone mineral density between left and right femurs in the control group(P>0.05).These results imply that the model of disuse osteoporosis by ankle-tail fixation is established successfully.Disuse osteoporosis can promote the production of CTx further reducing bone mineral density;CTx is positively correlated with the degree of bone loss,so it can be used for therapeutic assessment and diagnosis of osteoporosis.

关 键 词：失用性骨质疏松症 Ⅰ型胶原羧基端交联肽 骨密度 骨吸收 组织构建 骨质疏松 肽类 骨密度 组织工程 

分 类 号：R318[医药卫生—生物医学工程]