跑台运动和益生菌干预对大鼠不同组织细胞膜的保护作用  

Protective effects of probiotics on the cell membrane in different tissues of rats after treadmill exercise

作　　者：莫伟彬[1,2] 周琰 杨衍滔[1] Mo Wei-bin;Zhou Yan;Yang Yan-tao(Sport School of Guangxi Normal University,Guilin 541004,Guangxi Zhuang Autonomous Region,China;State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources,Ministry of Science and Technology of China,Guilin 541004,Guangxi Zhuang Autonomous Region,China;Department of Clinical Medicine,Shanghai General Hospital of Shanghai Jiao Tong University,Shanghai 201600,China)

机构地区：[1]广西师范大学体育学院,广西壮族自治区桂林市541004 [2]药用资源化学与药物分子工程国家重点实验室,广西壮族自治区桂林市541004 [3]上海交通大学附属第一人民医院临床药学科,上海市201600

出　　处：《中国组织工程研究》2018年第12期1877-1882,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学资金资助项目(21562006;31060121);教育发展基金会"教师成长基金"项目(EDF2016005);广西教育厅科研项目(2018KY0090);国家重点实验室开放项目(CMEMR2012-B04)~~

摘　　要：背景:长时间的力竭性运动应激引起机体不同组织器官免疫功能发生变化及益生菌干预引起机体机制尚不明确。目的:通过力竭性运动和益生菌干预观察大鼠不同组织器官形态学变化和细胞膜的保护作用。方法:40只SD雄性大鼠随机分为安静对照组(正常对照),运动对照组,安静给药组,运动给药组。运动对照组与运动给药组进行6周力竭训练,速度为19.3 m/min,坡度为5°,每天训练1次,6 d/周。安静给药组与运动给药组每日灌胃浓度为10~7CFU/mL益生菌溶液10.0 mL/(kg.d)。6周力竭训练结束后,通过光学显微镜下观察其病理组织学变化和测定大鼠肝、肠和胃组织Na^+/K^+-ATP酶、Ca^(2+)/Mg^(2+)-ATP酶、T-ATP酶的指标。结果与结论:(1)力竭运动引起大鼠肝、胃和肠组织结构紊乱、肿胀和有炎症细胞浸润现象,各组织中Na^+/K^+-ATP酶、Ca^(2+)/Mg^(2+)-ATP酶、T-ATP酶的活性有所下降;(2)通过服用益生菌溶液后大鼠肝、胃和肠组织结构排列紧密、清晰、无明显的充血和水肿,但仍有少量的炎症细胞和轻微的溃疡,各组织中Na^+/K^+-ATP酶、Ca^(2+)/Mg^(2+)-ATP酶、T-ATP酶的活性增高;(3)结果表明,益生菌溶液能够保护长时间力竭运动引起肝、胃和肠组织结构的变化和增高各组织中Na^+/K^+-ATP酶、Ca^(2+)/Mg^(2+)-ATP酶、T-ATP酶的活性来保证细胞膜的完整性及流动性。BACKGROUND:Changes in the immune function of different tissues after long-time exhaustive exercise stress and probiotics intervention are still unclear.OBJECTIVE:To observe the morphological changes and cell membrane protective effects of different tissues and organs in rats after exhaustive exercise and probiotics intervention.METHODS:Forty Sprague-Dawley male rats were randomly allocated into quiet control group,exercise control group,quiet administration group and exercise administration group.Rats in the exercise control and exercise administration groups underwent 6 weeks of exhaustive training,at a speed of 19.3 m/min,slope of 5o,once daily,6 days weekly.Rats in the quiet administration and exercise administration groups were given 107 CFU/mL probiotics via gavage,10.0 mL/(kg?d).The histopathological changes were observed under light microscope and Na+/K+-ATPase,Ca2+/Mg2+-ATPase and T-ATPase in the liver,intestine and stomach were tested after 6 weeks of training.RESULTS AND CONCLUSION:After exhaustive exercise,the liver,stomach and intestinal tissue presented with structure disorders,swelling and inflammatory cell infiltration and an decrease in the activity of Na+/K+-ATPase,Ca2+/Mg2+-ATPase and T-ATPase.After probiotic solution administrated,the structure of liver,stomach and intestine arranged closely and clearly,with no obvious congestion and edema.However,there were a few inflammatory cells and slight ulcers,and the activities of Na+/K+-ATPase,Ca2+/Mg2+-ATPase and T-ATPase were increased.These findings show that probiotic solution can protect the liver,stomach and intestinal tissue from injury caused by long-time exhaustive exercise,and maintain the integrity and fluidity of cell membrane by increasing Na+/K+-ATPase,Ca2+/Mg2+-ATPase and T-ATPase activities.

关 键 词：益生菌 力竭运动 ATP酶 组织:组织构建 有益菌种 运动医学 腺苷三磷酸 组织工程 

分 类 号：R318[医药卫生—生物医学工程]