载丹皮酚透钙磷石骨水泥的制备与表征  

Preparation and characterization of paeonol loaded brushite calcium phosphate cement

作　　者：李晓玉 李光大[2] 赵三团[3] 李永凯 赵朋超[2] 彭欢 Li Xiao-yu;Li Guang-da;Zhao San-tuan;Li Yong-kai;Zhao Peng-chao;Peng Huan(Central Lab,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;College of Medical Technology and Engineering,Henan University of Science and Technology,Luoyang 471023,Henan Province,China;College of Material Science and Engineering,Henan University of Science and Technology,Luoyang 471023,Henan Province,China)

机构地区：[1]首都医科大学附属北京世纪坛医院中心实验室,北京市100038 [2]河南科技大学,医学技术与工程学院,河南省洛阳市471023 [3]河南科技大学,材料科学与工程学院,河南省洛阳市471023

出　　处：《中国组织工程研究》2018年第10期1499-1505,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81402225,21461009)

摘　　要：背景:丹皮酚是传统骨科良药的活性成分,镇痛消肿,可促进受伤骨组织愈合。透钙磷石骨水泥生物相容性好,且可在体内完全降解,大量研究关注改性以使其更适合临床应用。目的:在透钙磷石骨水泥中负载丹皮酚,制备丹皮酚/透钙磷石骨水泥可注射支架材料,研究丹皮酚的加入对骨水泥固化时间、可注射性、抗压强度、缓释性能、抗菌性能及细胞亲和性能的影响。方法:以硝酸钙和磷酸氢二铵为原料湿法制备磷酸三钙,并1 000℃煅烧成β-磷酸三钙;将壳聚糖溶解于柠檬酸溶液制备固化液;将β-磷酸三钙、磷酸二氢钙和丹皮酚的混合粉末与固化液均匀调拌制备透钙磷石骨水泥。测试骨水泥的固化时间、可注射性、理化结构、抗溃散性、抗压强度、降解性能、药物缓释性能、抗菌性能和细胞亲和性。结果与结论:随丹皮酚含量增加,骨水泥固化时间延长,均在12-17 min之间,但抗溃散性、可注射性、抗压强度和晶相与基团组成均不受显著影响;材料降解性增强,药物释放浓度增加。抑菌圈实验结果显示,复合骨水泥对大肠杆菌生长具有一定抑制作用,但对金黄色葡萄球菌不敏感。将Hep G2细胞悬液滴加于材料表面并培养3 d后,通过扫描电镜可清晰观察到细胞伸出伪足,并能够在材料上黏附生长。BACKGROUND:Paeonol is an active ingredient of traditional orthopedic drugs,exhibiting pain easing,detumescence and promotion of bone healing.The brushite calcium phosphate cement possesses good biocompatibility,which can be completely degraded in the body.A large number of studies have addressed the modification of brushite calcium phosphate cement to make it more suitable for clinical applications.OBJECTIVE:To study the setting time,syringeability,compressive strength,drug delivery ability,antibacterial property and cell affinity of brushite calcium phosphate cement with the addition of paeonol.METHODS:The tricalcium phosphate was synthesized by calcium nitrate and diammonium hydrogen phosphate,followed by being calcined intoβ-tricalcium phosphate at 1 000℃.Chitosan was dissolved in citric acid solution to prepare the liquid phase.The composite bone cement was prepared by mixing paeonol,β-tricalcium phosphate and monocalcium phosphate with the liquid phase.The setting time,syringeability,phase composition,anti-collapsibility,compressive strength,degradation property,drug delivery ability,antibacterial property and cell affinity of the composite bone cement in vitro were evaluated.RESULTS AND CONCLUSION:The setting time of bone cement was 12-17 minutes,which was prolonged with the increase of paeonol release.Moreover,the loaded paeonol showed no significant effect on the phase composition,syringeability,anti-collapsibility,and compressive strength of the compound.However,the degradation rate and drug release content were significantly enhanced with the increase of paeonol release.The inhibition zone experiments showed that the paeonol loaded cement inhibited the growth of Escherichia coli,but was not sensitive to Staphylococcus aureus.HepG2 cells could adhere and proliferate on the material surface after 3 days co-culture,with clear cell pseudopodia arising from the cell surface under the scanning electron microscope.

关 键 词：透钙磷石骨水泥 丹皮酚 可注射性 抗溃散性 药物缓释 抗菌性能 细胞亲和性 骨生物材料 磷酸钙类 丹皮酚 组织工程 

分 类 号：R318[医药卫生—生物医学工程]