β-磷酸三钙复合成骨细胞特异性多肽植入拔牙窝位点保存牙槽骨  

Implantation of beta-tricalcium phosphate combined with osteoblast-specific peptides for alveolar bone preservation in extraction sockets

作　　者：庄艳琴 陈慧敏[1] 吴齐越 王泽华 吴明月[1] Zhuang Yan-qin;Chen Hui-min;Wu Qi-yue;Wang Ze-hua;Wu Ming-yue(College&Hospital of Stomatology,Anhui Medical University,Hefei 230032,Anhui Province,China)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,安徽省合肥市230032

出　　处：《中国组织工程研究》2018年第10期1517-1522,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81170993)

摘　　要：背景:课题组前期研究发现成骨细胞特异性多肽能促进兔颅骨缺损的修复再生,而β-磷酸三钙作为支架材料负载成骨细胞特异性多肽,二者不仅功能互补,且能充分发挥骨诱导及骨传导的双重效应。目的:以β-磷酸三钙骨粉为支架负载成骨细胞特异性多肽,检测成骨细胞特异性多肽在兔下前牙拔牙位点保存实验中的作用及对牙槽骨重建的影响。方法:将27只新西兰大白兔随机分成3组(n=9),拔除右侧下颌中切牙,建立拔牙窝位点保存动物模型。实验组植入β-磷酸三钙骨粉/成骨细胞特异性多肽复合物,材料组植入单纯β-磷酸三钙骨粉,空白组不植入任何材料及药物,造模后4,8及12周每组各处死3只大白兔,制备组织标本,通过大体观察、组织形态学测量以及影像学测量评价拔牙窝愈合情况。结果与结论:(1)影像学结果,造模后4,8及12周剩余牙槽骨的相对长度为:实验组>材料组>空白组,差异有显著性意义(P<0.05)。锥形束CT可见:随着时间推移,造模后4及8周实验组和材料组材料逐渐降解,实验组新生骨量较材料组和空白组明显,12周时实验组拔牙窝基本完成重建,材料组及空白组仍有部分骨缺损;(2)组织形态学表明:造模后4周实验组见明显骨沉积线,骨小梁增宽;材料组及空白组新生骨较少。造模后8周实验组内可见少量未降解的支架材料,见成熟的板层状骨,材料组新生骨量增多,空白组成骨细胞明显。造模后12周实验组拔牙窝内骨改建基本完成,材料组仍可见少量支架材料,见致密板状新骨;空白组新生骨趋于成熟,见明显板层状结构;(3)结果表明,成骨细胞特异性多肽能够有效保存拔牙窝剩余牙槽骨长度,促进新骨形成,具有保存拔牙位点的作用。BACKGROUND:Previous findings show that osteoblast-specific peptides can promote the repair and regeneration of skull defects in rabbits,andβ-tricalcium phosphate(β-TCP)is used as a scaffold to carry osteoblast-specific polypeptides.Both of them not only complement each other,but also fully exert dual effects of osteoinduction and bone conduction.OBJECTIVE:To investigate the effect of osteoblast-specific peptide on the preservation of the anterior tooth extraction site in rabbits,and to study the effect on the alveolar bone remodeling.METHODS:Twenty-seven New Zealand white rabbits were randomly divided into three groups(n=9 per group),and the right mandibular incisors were removed to establish the animal models of tooth extraction.β-TCP/osteoblast-specific peptide compounds were implanted in the experimental group,and pureβ-TCP meal was implanted into the material group.The blank control group had no implantation.Three rabbits from each group were scarified at 4,8 and 12 postoperative weeks,and tissue samples were prepared for gross observation,histomorphology measurements,and radiographic measurements of extraction socket healing.RESULTS AND CONCLUSION:(1)Imaging results showed that the relative length of residual alveolar bone after modeling was ranked as follows:the experimental group>the material group>the blank control group,and the difference was statistically significant among groups(P<0.05).Cone-beam CT findings in the three groups changed as time went on.At 4 and 8 postoperative weeks,the implanted materials in the experimental and material groups gradually degraded;the bone mass in the experimental group was significantly higher than that in the material and blank control groups.At 12 postoperative weeks,the experimental group had basically completed the reconstruction of tooth socket,but there were still some bone defects in the material and blank control groups.(2)Histomorphological findings showed that at 4 postoperative weeks,the experimental group exhibited obvious bone deposition lines,and the

关 键 词：成骨细胞特异性多肽 β-磷酸三钙骨粉 材料植入 复合支架 牙槽骨缺损 组织重建 位点保存 国家自然科学基金 植入物 实验性 拔牙 组织支架 成骨细胞 组织工程 

分 类 号：R318[医药卫生—生物医学工程]