复合明胶甲基丙烯基水凝胶在骨组织工程中的应用及前景  

Application prospects of compound gelatin-methacryloyl hydrogel in bone tissue engineering

作　　者：刘肇兴 吴兴 林欢欢 刘沙 陈向军 Liu Zhao-xing;Wu Xing;Lin Huan-huan;Liu Sha;Chen Xiang-jun(School of Graduate,Inner Mongolia Medical University,Hohhot 010030,Inner Mongolia Autonomous Region,China;Department of Burn and Plastic Surgery,the 253rd Hospital of Chinese PLA,Hohhot 010030,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古医科大学研究生学院,内蒙古自治区呼和浩特市010030 [2]解放军第253医院烧伤整形科,内蒙古自治区呼和浩特市010030

出　　处：《中国组织工程研究》2018年第10期1593-1598,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:复合明胶甲基丙烯基水凝胶己被证实其在骨、软骨、心肌、血管等组织再生方面具有独特优势。目的:归纳总结复合明胶甲基丙烯基水凝胶在骨组织工程中的研究新进展。方法:检索PubM ed数据库和CNKI数据库2010至2017年发表的文献,中文检索关键词为"明胶,甲基丙烯酸,水凝胶,羟基磷灰石,骨组织工程,骨再生,种子细胞,微环境",英文检索关键词为"gelatin,methacrylamide,hydrogel,hydroxyapatite,bone tissue engineering,bone regeneration,seed cells,micro environment"。结果与结论:可通过特定方式在复合明胶甲基丙烯基水凝胶中加入无机成分,以光刻、微流体、微成模及3D打印技术制备出仿生组织工程骨,使其在结构、力学和生物学特性上与天然骨组织相似,并具有成骨性。将复合明胶甲基丙烯基水凝胶与加载基因修饰的种子细胞置于一定条件下进行三维培养,经相关生物特性检测,发现植入支架中的种子细胞生长状态良好,并有与成骨再生性及血管再生性相关的基因表达。因此复合明胶甲基丙烯基水凝胶具有良好的体外成骨效果,是优良的骨组织工程材料。BACKGROUND:Compound gelatin-methacryloyl(GelMA)hydrogel has been shown to have unique advantages in bone,cartilage,myocardium,and vascular regeneration.OBJECTIVE:To summarize the novel progress of compound GelMA hydrogel for bone tissue engineering.METHODS:PubMed database and CNKI database from 2010 to 2017 were searched by using the keywords of“gelatin,methacrylamide,hydrogel,hydroxyapatite,bone tissue engineering,bone regeneration,seed cells,microenvironment”in Chinese and English,respectively.RESULTS AND CONCLUSION:Inorganic components can be added in a specific way into the compound GelMA hydrogel to prepare tissue-engineered bone using photolithography,microfluidics,microfabrication and 3D printing techniques.The prepared tissue-engineered bone has similar structural,mechanical and biological properties to natural bone tissue,and importantly,it has osteogenic ability.Gene-modified seed cells that are co-cultured with the compound GelMA hydrogel in a 3D environment are found to grow well and express some genes related to bone regeneration and vascular regeneration.Therefore,the compound GelMA hydrogel has a good osteogenesis effect in vitro,which is an excellent material for bone tissue engineering.

关 键 词：生物材料 明胶 甲基丙烯酸 水凝胶 羟基磷灰石 骨组织工程 骨再生 种子细胞 微环境 明胶 聚甲基丙烯酸类 水凝胶 骨再生 组织工程 

分 类 号：R318[医药卫生—生物医学工程]