3D生物打印在组织/器官类似物制造领域的应用  

Three-dimensional bioprinting of tissue/organ analogues: a review on techniques, materials and processes

作　　者：王雪欣 张明谏[2] 李小兵[2] 韩维鑫 李金桥 Wang Xue-xin;Zhang Ming-jian;Li Xiao-bing;Han Wei-xin;Li Jin-qiao(Department of Plastic and Burn Surgery,the Clinical School of Tianjin Medical University,Tianjin 300070,China;Department of Plastic and Burn Surgery,Tianjin First Central Hospital,Tianjin 300192,China;Department of Plastic and Burn Surgery,Tianjin Third Central Hospital,Tianjin 300170,China)

机构地区：[1]天津医科大学一中心临床学院,天津市300070 [2]天津市第一中心医院整形与烧伤外科,天津市300192 [3]天津市第三中心医院美容整形科,天津市300170

出　　处：《中国组织工程研究》2018年第10期1611-1617,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:3D生物打印技术在组织工程领域具有巨大潜力,有望利用活细胞和活性生物材料构建具有良好生物组织相容性和生物学功能的简单组织和器官。目的:分析3D生物打印技术和各种生物材料的特点,探讨其在相关组织/器官类似物制造中的应用。方法:应用计算机检索1998至2017年Pub Med数据库、Web of Science数据库、Medline数据库、万方医学网数据库有关3D生物打印技术和应用的相关文献。以"3D生物打印,3D生物打印技术,生物材料,组织工程"为中文主题检索词;以"3D bioprinting,3D bioprinting technology,biomaterial,tissue engineering"为英文主题检索词,检索题目和摘要同时包含上述检索词的文献共88篇,排除重复性研究和无关研究,最终纳入47篇文献进一步分析。结果与结论:目前应用比较广泛的3D生物打印技术主要包括喷墨式技术(热喷墨与压电喷墨)、压力辅助打印技术、激光辅助打印技术、光固化立体印刷技术(单光子和双光子)。生物墨的构成除了原生活细胞,还包括天然聚合物和合成聚合物,各有优劣势。3D生物打印含有活细胞的组织/器官类似物在组织工程领域显示出巨大的潜力,尽管已有较广泛的研究,但目前的印刷技术只能完成相对简单的组织结构和生物功能,含有活细胞的3D组织/器官生物打印仍然处于探索阶段。BACKGROUND:Three-dimensional(3D)bioprinting technology has a huge potential in the tissue engineering field,which is expected to create simple tissue/organ analogues with good biological histocompatibility and biological functions by using living cells and biomaterials.OBJECTIVE:To analyze the characteristics of 3D bioprinting technology and all kinds of biomaterials,and to explore its application in the preparation of tissues/organs analogues.METHODS:Relevant articles published from 1998 to 2017 were searched in PubMed,Web of Science,MEDLINE,and WanFang databases.The keywords were“3D bioprinting,3D bioprinting technology,biomaterial,tissue engineering”in English and Chinese,respectively.A total of 88 articles were initially searched and 47 eligible articles were finally reviewed in accordance with the inclusion and exclusion criteria.RESULTS AND CONCLUSION:3D bioprinting techniques mainly include inkjet technique(thermal inkjet and piezoelectric inkjet),pressure-assisted technique,laser-assisted technique,and stereolithography technique(single-photon-based and two-photon-based).The bio-ink consists of living cells,natural polymers and synthetic polymers.3D bioprinting has exhibited a huge potential in the manufacture of living cell-containing tissue/organ analogues.Despite the fact that it has been widely studied,currently used 3D bioprinting techniques can only be used to prepare relatively simple structures with simple biological functions.Research on the specific tissue/organ analogues with living cells are still in its infancy.

关 键 词：3D生物打印 3D生物打印技术 生物材料 组织/器官类似物 组织工程 计算机辅助设计 综述 组织工程 

分 类 号：R318[医药卫生—生物医学工程]