实验室饲养和野外生存条件下东方田鼠肠道菌群的多样性  

Comparative analysis of intestinal flora of Microtus fortis living under laboratory feeding and wild survival conditions

作　　者：冯洁 沈志敏 王胜昌 林金杏 柏熊 谢建云 FENG Jie;SHEN Zhimin;WANG Shengchang;LIN Jinxing;BO Xiong;XIE Jianyun(Shanghai Laboratory Animal Research Center,Shanghai 201203,China;Shanghai Xipuer-Bikai Laboratory Animal Limited Company,Shanghai 201203)

机构地区：[1]上海实验动物研究中心,上海201203 [2]上海西普尔-必凯实验动物有限公司,上海201203

出　　处：《中国实验动物学报》2018年第2期188-194,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划(No.2015BAI09B04)~~

摘　　要：目的探讨实验室饲养和野外生存条件下东方田鼠肠道菌群的分布和差异。方法应用高通量测序技术测定实验室饲养和野外捕捉东方田鼠的肠道细菌16S rDNA-V4-V5区序列,统计操作分类单元(OTUs)数量,分析菌群物种丰度、分布及差异性。结果稀疏曲线表明取样数量合理,测序充分。在门水平,实验室饲养和野外生存的肠道菌群分布和占比基本相似,实验组有1个独有的门,即黏胶球形菌门(Lentisphaerae),野生组有3个独有的门,分别是梭杆菌门(Fusobacteria)、奇古菌门(Thaumarchaeota)和未分类的门,丰度差异最大的为软壁菌门(Tenericutes)。在属水平,野外东方田鼠肠道细菌较实验室饲养的丰富,丰度差异最大的为瘤胃球菌属(Ruminococcus)。结论获得了实验室饲养和野外生存条件下东方田鼠肠道菌群结构和差异性,进一步充实了东方田鼠基础生物学数据。Objective To compare the differences of bacterial distribution of intestinal flora in Microtus fortis living under laboratory feeding and wild survival conditions.Methods The 16S rDNA-V4-V5 region of bacteria in the ileocecal contents from Microtus fortis raised in lab and captured in wild were measured by high-throughput sequencing.The number of operational taxonomic units(OTUs)were sorted and calculated,and the species abundance and distribution and difference were analyzed.Results The rarefaction curves indicated that adequate sampling was achieved.At the phylum level,the distribution of intestinal flora between two groups was similar.The experimental group had a unique phylum,Lentisphaerae.The wild type group had 3 unique phylums,Fusobacteria,Thaumarchaeota and an unclassified phylum.At the genus level,the kind of intestinal flora in the wild type group was more abundant than the experimental group.Ruminococcus is the largest differential genus.Conclusions The microbial community structure and differences of Microtus fortis living under different conditions are obtained.It may further enrich the basic biology data of Microtus fortis.

关 键 词：东方田鼠 肠道菌群 16S RDNA 多样性 

分 类 号：Q95-33[生物学—动物学]