高效液相色谱法测定鼠药中安妥的含量  

Determination of Antu in Raticide by HPLC

作　　者：李军德[1] LI Junde(Hospital of Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学校医院,山东青岛266109

出　　处：《青岛农业大学学报（自然科学版）》2018年第1期66-68,72,共4页Journal of Qingdao Agricultural University(Natural Science)

摘　　要：建立了利用高效液相色谱法分析测定老鼠药中安妥的新方法。通过对提取剂种类、流动相的组成、柱温、流动相流速和紫外检测波长等色谱条件的优化,降低了方法的检测限,安妥的检测限为0.1ng/mL(信噪比为3∶1),加标回收率为101.7%。用外标法定量时,线性相关系数0.999 9。该方法用于实际鼠药样品的测定,简单、快速、准确,灵敏度高。In the present work,a method was developed for the determination of antu in raticide samples by high performance liquid chromatography.The effects of extractants,proportion of mobile phase,column temperature,flow rate of mobile phase,and ultraviolet detection wavelength were optimized.The detection limit of 0.1 ng/mL(S/N=3∶1)and the spiking recovery of 101.7%were obtained for antu.The correlation coefficient for antu was perfect in the function.The results show that the method is reliable,fast and sensitive on the real samples.

关 键 词：安妥 高效液相色谱 色谱图 分析检测 

分 类 号：O657.7[理学—分析化学]