sEphB4对糖尿病视网膜病变兔模型视网膜血管内皮细胞EphB4/EphrinB2和VEGF表达的影响  

Effect of sEphB4 on the expression of EphB4/EphrinB2 and VEGF in retinal vascular endothelial cells in rabbit model of diabetic retinopathy

作　　者：颜坚[1] 彭细峰[1] 蔡玉莲[1] 邓江涛[1] 张涛 YAN Jian;PENG Xifeng;CAI Yulian;DENG Jiangtao;ZHANG Tao(Longgang Central Hospital,Shenzhen 518116,China)

机构地区：[1]深圳市龙岗中心医院,广东深圳518116

出　　处：《中国医药科学》2018年第7期41-44,共4页China Medicine And Pharmacy

基　　金：广东省深圳市科技研发资金知识创新计划(JCYJ20150403091931178)

摘　　要：目的探讨s Eph B4对糖尿病视网膜病变兔模型视网膜血管内皮细胞Eph B4/Ephrin B2和VEGF表达的影响。方法选8~10周的雄兔8只为实验对象,建立糖尿病视网膜病变兔模型。将所有DR兔的右眼作为药物干预组,为药物干预组,分别予s Eph B4 200、500、1000μg各50μL玻璃体腔注入。左眼作为对照组,予以生理盐水50μL玻璃体腔注入。每月注射1次,治疗6个月后,应用检测兔眼视网膜及玻璃体增殖膜Eph B4、Ephrin B2和VEGF的表达。结果随着s Eph B浓度的不断增加,Eph B4、Ephrin B2、VEGF水平逐渐降低,且均明显低于对照组,差异有统计学意义(P<0.05)。结论 s Eph B4可抑制糖尿病视网膜病变兔视网膜新生血管内皮细胞Eph B4/Ephrin B2和VEGF的表达。Objective To investigate the effect of sEphB4 on the expression of EphB4/EphrinB2 and VEGF in retinal endothelial cells of diabetic retinopathy rabbit model.Methods The diabetic retinopathy model was established in 8 male rabbits(8-10 weeks old).The right eyes of all Dr rabbits were used as drug intervention group,and were injected with sEphB4 200μg,500μg,1000μg each 50μL vitreous cavity respectively.The left eye as control group was injected with normal saline 50μL vitreous cavity.The expression of EphB4/EphrinB2 and VEGF in retina and vitreous proliferative membrane were detected after 6 months of treatment.Results The level of EphB4,EphrinB2 and VEGF was gradually decreased with the increasing of sEphB,which was significantly lower than that of the control group(P<0.05).Conclusion sEphB4 can inhibit the expression of EphB4/EphrinB2 and VEGF in retinal neovascular endothelial cells in rabbits with diabetic retinopathy.

关 键 词：糖尿病视网膜病变兔模型 视网膜血管内皮细胞 EphB4/EphrinB2 VEGF 

分 类 号：R276.7[医药卫生—中医五官科学]