实时直接分析-质谱法快速检测饮料和尿液中的γ-羟基丁酸  

Rapid Determination of γ-Hydroxybutyric Acid in Beverage and Urine by Direct Analysis in Real-time Mass Spectrometry

作　　者：刘佳蓉 黄忠平[1] 刘会君[1] 王丽丽[1] 刘春胜 任一平 史鸿鑫[1] LIU Jia-Rong;HUANG Zhong-Ping;LIU Hui-Jun;WANG Li-Li;LIU Chun-Sheng;REN Yi-Ping;SHI Hong-Xin(College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310014,China;ASPEC Technologies Limited,Beijing 100101,China;National Center for Food Safety Risk Assessment Application Technology Cooperation Center,Yangtze Delta Region Institute of Tsinghua University,Jiaxing 314006,China)

机构地区：[1]浙江工业大学化学工程学院,杭州310014 [2]华质泰科生物技术有限公司,北京100101 [3]国家食品安全风险评估中心应用技术合作中心清华大学长三角研究院,嘉兴315000

出　　处：《分析化学》2018年第5期698-703,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.51503182);浙江省分析测试基金(No.2017C37064);浙江省家具检测技术研究重点实验室资助项目(No.2016J01)资助~~

摘　　要：建立了一种实时直接分析-质谱法(DART-MS)用于饮料(水、碳酸饮料、啤酒)和尿液中γ-羟基丁酸(GHB)快速检测。样品经甲醇-水(1∶1,V/V)溶液稀释后,在负离子模式下,以选择离子扫描(SIR)模式进行直接定量分析。离子化气体温度为350℃,进样速率为0.5 mm/s。针对水样、碳酸饮料、啤酒、尿液样品,本方法的检出限(S/N=3)为1~2μg/m L,定量限(S/N=10)为3~5μg/m L。标准曲线线性相关系数为0.9899~0.9980,加标回收率为80.8%~115.2%,相对标准偏差为1.9%~12.8%。本方法具有样品前处理简单、分析速度快、成本低等优点,有望在大批量饮料和尿液样品的快速筛查分析中发挥作用。A method for rapid determination ofγ-hydroxybutyric acid(GHB)in beverages(water,sodas,beer)and urine was established by direct analysis real-time mass spectrometry(DART-MS).Samples were analyzed directly after dilution with mixture of methanol and water(1∶1,V/V).Instrument parameter settings were optimized to obtain the sensitive and accurate determination of GHB.At the sample introduction speed of 0.5 mm/s,high intensity of[M-H]0x0E?Symbolm@@0x0F ions for GHB were observed in the negative ion and selection ion monitoring mode by utilization of high purity helium gas at 350℃.For different samples of water,sodas,beer and urine,the limits of detection(LODs)(S/N=3)were in the range of 1-2μg/mL,while the limits of quantification(LOQs)(S/N=10)were in the range of 3-5μg/mL.The linear correlation coefficients of the standard curves with different sample matrixes were between 0.9899 and 0.9980.The recoveries were in the range of 80.8%-115.2%with the relative standard deviations of 1.9%-12.8%.With its rapid analysis and simple pretreatment steps,the method is expected to have a strong advantage in the rapid screening analysis of large quantities of beverage and urine.

关 键 词：实时直接分析-质谱法 Γ-羟基丁酸 快速筛查 

分 类 号：O657.63[理学—分析化学]