汞胁迫对红裸须摇蚊幼虫抗氧化酶活性的影响  

Effect of Hg( Ⅱ) Exposure on Activities of Antioxidant Enzyme in the Larva of Propsilocerus akamusi

作　　者：耿飞飞[1] 于洪贤 刘曼红[1] 孟瑶[1] Geng Feifei;Yu Hongxian;Liu Manhong;Meng Yao(Northeast Forestry University,Harbin 150040,P.R.China)

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2018年第4期97-100,共4页Journal of Northeast Forestry University

基　　金：中央高校基本科研业务费专项资金项目(2572014EA07-03)

摘　　要：为评价Hg^(2+)对水生昆虫的毒理效应,将红裸须摇蚊(Propsilocerus akamusi)幼虫置于质量浓度为0、1.6、2.4、3.6、5.4、8.1 mg/L的溶液中,分别暴露6、24、48、72 h时取样,分析不同处理对红裸须摇蚊幼虫组织匀浆过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、谷丙转氨酶(GST)的影响。结果表明:(1)同一时间不同质量浓度的Hg^(2+)处理,对4种酶活性影响的LSD法多重比较检验结果显示,暴露6、48、72 h时,Hg^(2+)对CAT活性都没有明显差异(p>0.05);仅在暴露24 h时,CAT活性在对照组与其他质量浓度处理组之间的差异达到了显著性水平(p<0.05)。(2)暴露6、24、48 h时,各处理组对SOD活性都没有明显差异;仅在暴露72 h时,SOD活性各处理组之间的差异达到了显著性水平(p<0.05)。(3)各时间各处理组的POD活性,都没有明显差异(p>0.05)。(4)暴露48、72 h时,各质量浓度Hg^(2+)对GST活性都没有明显差异(p>0.059);暴露6、24 h时,GST活性在对照组与其他质量浓度处理组之间的差异达到了显著性水平(p<0.05)。(5)CAT、SOD、GST活性对Hg^(2+)具有较高的敏感性,可以作为生物化学标志物检测水体中Hg^(2+)的早期污染;POD活性呈相对复杂的变化,POD不能有效地用来检测水环境早期Hg^(2+)的污染。We evaluated the toxicity effect of Hg 2+on aquatic insects,Propsilocerus akamusi larvas were put into the Hg 2+solution with the concentration of 0,1.6,2.4,3.6,5.4 and 8.1 mg/L.We studied the activities effect of CAT,POD,SOD and GST on P.akamusi larvas homogenate which were exposed for 6,24,48 and 72 h.The LSD multiple comparison test result for different concentration of Hg 2+to four enzymes in the same time showed:①There was no significant difference in CAT activity for 6,48,and 72 h(p>0.05).Only the 24 h was obviously different from the control groups and other treatments(p<0.05).The 6,24 and 48 h had no significant difference in SOD activity.②Only the 72 h had significant difference from other treatments(p<0.05).③All treatment had no significant difference in POD activity all the time(p<0.05).④The 48 and 72 h had no significant difference in GST activity(p>0.05),and the differences between the control group and the other treated groups reached a significant level at 6 and 24 h exposed Hg 2+(p<0.05).⑤CAT,SOD and GST were high sensitivity in Hg 2+.They may be served as a biochemical marker in monitoring pollution of Hg 2+early.While the POD activity was complex,thus it wasn’t an effective target to test the early water pollution about Hg 2+.

关 键 词：水生昆虫 红裸须摇蚊 昆虫毒理效应 汞胁迫 抗氧化酶活性 

分 类 号：Q969.442.6[生物学—昆虫学] Q965.9