不同浓度半胱氨酸对幼龄陶寒杂种羊卵母细胞发育的影响  

Effect of different concentrations of cysteine on the development of oocytes in young pottery and cold hybrid sheep

作　　者：罗惠娣[1] 毛杨毅[1] 周胜花[1] 郭慧慧[1] 李春艳[1] 李希[1] 张丽[1] 吴苏君[1] 薛丽娜[1] 党文庆[1] Luo Huidi;Mao Yangyi;Zhou Shenghua;Guo Huihui;Li Chunyan;Li Xi;Zhang Li;Wu Sujun;Xue Lina;Dang Wenqing(Institute of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences,Shanxi Taiyuan 030032)

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032

出　　处：《现代畜牧兽医》2018年第4期7-11,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：国家绒毛用羊产业技术体系建设专项(CARS-39-24);山西省科技攻关项目(20120311024-1);山西省科技创新团队项目(201705D131028-20);山西省"三区"人才专项基金(2017Sy128)

摘　　要：为了探讨半胱氨酸对幼龄陶寒杂种羔羊卵母细胞发育的影响,试验采用激素诱导4~8周龄陶寒杂种羔羊获得624枚A、B级卵丘卵母细胞复合体(COCs),分别在含不同浓度半胱氨酸的成熟液里成熟培养24 h,对照组(0μmol/L)、试验Ⅰ组(100μmol/L)、试验Ⅱ组(150μmol/L)和试验Ⅲ组(200μmol/L),经体外受精检测卵母细胞发育效果。结果表明:试验Ⅰ组、Ⅱ组和Ⅲ组成熟卵母细胞的卵裂率分别为60.9%、62.82%和64.1%,比对照组的卵裂率59.62%分别高1.28、3.2和4.48个百分点(P>0.05),试验Ⅰ组、Ⅱ组和Ⅲ组成熟卵母细胞的囊胚率分别为21.79%、23.08%和23.72%,比对照组的囊胚率18.59%分别高3.2、4.49和5.13个百分点(P>0.05)。说明成熟液里添加一定量半胱氨酸有利于卵母细胞成熟和发育。In order to explore the effect of cysteine on the development of oocytes in young pottery and cold hybrid sheep,624 A and B grade oocyte complexes(COCs)of 4-8-week-old young pottery and cold hybrid sheep with hormone-induced were tested in groups with different concentrations of cysteine,control group(0μmol/L),GroupⅠ(100μmol/L),GroupⅡ(150μmol/L)and GroupⅢ(200μmol/L),after 24 h maturation in mature liquid IVF was carried out to investigate the development competence of oocytes.The results showed that the cleavage rates were 60.9%,62.82%and 64.1%in GroupⅠ,GroupⅡand groupⅢrespectively,which were 1.28,3.2 and 4.48%higher than that in the control group(59.62%)(P>0.05).The blastocyst rates of groupⅠ,GroupⅡand groupⅢwere 21.79%,23.08%and 23.72%respectively compared with the rate of control group 18.59%.It is suggested that the addition of a certain amount of cysteine in the mature liquid is beneficial to the maturation and development of oocyte.

关 键 词：半胱氨酸 幼龄陶寒杂种羊 卵母细胞 发育 

分 类 号：S826[农业科学—畜牧学]