额叶p-mTOR异常表达与外伤性癫痫发生的相关研究  

Assessment of the correlation between posttraumatic epilepsy and the abnormal expression of p-mTOR in frontal lobe

作　　者：张艳丽[1] 吐尔逊.沙比尔 Zhang Yanli;Tuerxun·Shabier(Division of Rehabilitation Medicine,Department of Neurology,Heze Municiple Hospital in Shandong Province,Heze 274000,China)

机构地区：[1]菏泽市立医院神经内科康复医学病区,山东菏泽274000 [2]新疆医科大学第一附属医院神经内科,乌鲁木齐830054

出　　处：《中国康复》2018年第2期91-94,共4页Chinese Journal of Rehabilitation

基　　金：国家自然科学基金(81260180)

摘　　要：目的:探索外伤性癫痫(Posttraumatic epilepsy,PTE)发生与额叶p-mTOR异常表达的相关性。方法:将78只成年雄性SD大鼠分为正常对照组(A组)6只,生理盐水对照组(B组)和癫痫模型组(C组)各36只,C组右侧额叶注射FeCl_2造模。应用免疫组化技术检测造模后1h、24h、1周、2周、4周各组大鼠额叶皮质中p-mTOR(Ser2448)的动态表达。结果:A组额叶p-mTOR(Ser2448)表达的免疫组织化学染色,神经元胞质呈浅棕色,提示弱阳性表达;B组细胞染色程度较A组加深,p-mTOR(Ser2448)表达阳性细胞数增加;C组可见细胞结构紊乱,胞质呈明显的棕黄色,提示该蛋白在细胞质中呈强阳性表达。C组和B组比较,1h表达开始升高(t=-1.435,P=0.182),但差异无统计学意义,1周表达高峰(t=-4.073,P=0.002),2周降低(t=-2.614,P=0.026),4周时再次升高(t=-2.506,P=0.031),差异具有统计学意义(均P<0.05)。结论:PTE的发病机制可能与mTOR信号通路上pmTOR(Ser2448)的表达异常增加有关。Objective:To explore the correlation between the abnormal expression of p-mTOR in the frontal lobe and posttraumatic epilepsy(PTE).Methods:Adult male SD rats were randomly divided into control group(n=6),saline group(n=36)and epilepsy group(n=36).Models were established by injecting FeCl 2 into the right frontal lobe.Immunohistochemistry was used to detect the dynamic expression of p-mTOR(Ser2448)in the frontal cortex of rats at different time points(1 h,24 h,1 week,2 weeks,and 4 weeks after modeling).Results:As compared with the saline group,the expression of p-mTOR(Ser2448)in the frontal cortex of the epilepsy group began to increase after 1 h(t=-1.435,P=0.182),reached its maximum after 1 week(t=-4.073,P=0.002),reduced after 2 weeks(t=-2.614,P=0.026)and rose again after 4 weeks(t=-2.506,P=0.031).The difference was statistically significant.Conclusion:The pathogenesis of PTE may be related to the abnormal increase of the expression of p-mTOR(Ser2448)protein in the mTOR signaling pathway.

关 键 词：外伤性癫痫 mTOR通路 发病机制 

分 类 号：R49[医药卫生—康复医学] R742.1[医药卫生—临床医学]