PCR-RFLP定量检测川贝母真伪的研究  

Authentication of Fritillariae cirrhosae bulbus by PCR-RFLP

作　　者：赵仲麟[1] 常志远 袁超[1] 李瑞歌[1] 王成 王永 兰青阔 ZHAO Zhonglin;CHANG Zhiyuan;YUAN Chao;LI Ruige;WANG Cheng;WANG Yong;LAN Qingkuo(College of Sciences,Henan Agricultural University,Zhengzhou 450002,China;School of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,321004,China;Institute of Tianjin Agriculture Quality Standard and Testing Technology,Tianjin 300381,China)

机构地区：[1]河南农业大学理学院,河南郑州450002 [2]浙江师范大学化学与生命科学学院浙江金华321004 [3]天津市农业质量标准与检测技术研究所,天津300381

出　　处：《河南农业大学学报》2018年第2期249-253,共5页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(31100067)

摘　　要：建立了一个适合中药材川贝母的聚合酶链式反应-限制性内切酶(PCR-RFLP)的分子鉴定方法。以提取的DNA为模板进行PCR扩增和酶切反应,真品川贝母扩增产物含有Sam I酶切位点,在100~250 bp之间存在明确的2条酶切条带,而伪品贝母扩增序列中没有Sam I酶切位点不能被切出。此外,还基于PCR-RFLP技术针对川贝母真伪性进行了相对定量分析研究。结果表明,含量10%及以上的真品川贝母能被稳定检测出。In this study,a molecular identification method of polymerase chain reaction restriction endonuclease(PCR-RFLP)was developed for identification of Fritillariae cirrhosae bulbus.The extracted DNA was used as the template for PCR amplification,and the amplification products were digested with endonuclease.Because Fritillariae cirrhosae bulbus has Sam I cut site,2 distinct bands between 100 bp to 250 bp could be observed.However,the pseudo Fritillaria could not be cut.Based on the PCR-RFLP technique,the relative quantitative analysis standardization of Fritillariae cirrhosae bulbus was also studied.The results showed that products containing 10%or more authentic Fritillariae cirrhosae bulbus could be detected.

关 键 词：川贝母 分子鉴定 PCR-RFLP 

分 类 号：R927.1[医药卫生—药学]