中药催情散对持久黄体奶牛血清中生殖激素含量的影响  

Effect of Chinese traditional aphrodisiac powder on reproductive hormones in serum of dairy cows with persistent corpus luteum

作　　者：刘伟[1] 蒋瑞东 任书男 祁生旺[1] 李云章[1] 查斯图 LIU Wei;JIANG Ruidong;REN Shu’nan;QI Shengwang;LI Yunzhang;Chasitu(College of Veterinary,Inner Mongolia Agricultural University,Hohhot 010018,China;Balinzuoqi Agriculture and Animal Husbandry Bureau,the Inner Mongolia Autonomous Region,Chifeng 025450,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]内蒙古自治区巴林左旗农牧业局,赤峰025450

出　　处：《华中农业大学学报》2018年第3期68-74,共7页Journal of Huazhong Agricultural University

基　　金：内蒙古自治区科技计划项目(20130224)

摘　　要：为阐明中药催情散治疗奶牛持久黄体的作用机制,通过直肠及B超检查技术将确诊的36头持久黄体奶牛分为高、中、低剂量组和对照组,试验组灌服不同剂量的催情散,对照组灌服温性自来水,连续灌服4d。统计有效率并用酶联免疫吸附法(ELISA)对用药前及用药后不同时间点试验奶牛血清中促卵泡素(FSH)、促黄体素(LH)、雌二醇(E_2)、孕酮(P_4)的含量进行测定,并进行组间及组内比较。结果显示:高、中剂量组总有效率都为70%,低剂量组总有效率为30%,对照组总有效率为16.7%;高、中剂量组总有效率与低剂量组相比差异显著,与对照组相比差异极显著。从激素水平来看,高、中、低剂量组奶牛用药后血清中FSH、LH、E_2水平均呈现升高趋势,P4水平均呈现下降趋势,在个别时间点甚至达到极显著水平,其中高剂量组变化趋势最明显,其次为中剂量组;而对照组奶牛灌服自来水后血清中生殖激素含量在各采样点间差异不显著,但LH和E_2在用药后16~19d有小幅度的升高趋势。以上结果表明,中药催情散通过调节持久黄体奶牛血清中生殖激素水平来达到治疗目的,从4组奶牛血清中生殖激素变化水平、用药后治愈率和有效率来看,高剂量组治疗效果最佳。To elucidate the mechanism of the aphrodisiac powder for treating cow’s persistent corpus luteum,36 cows with persistent corpus luteum,which was detected through rectum and B ultrasound examination,were treated with high,medium,low doses of aphrodisiac powder and with warm water for the negative control for continuous 4 d.The efficiency of treatment was calculated and serum FSH,LH,E 2,P 4 were measured using enzyme-linked immunosorption method at different time points.The results showed that the total effective rate was 70%in the high and medium dose group,30%in the low dose group,and 16.7%in the negative control.The total effective rate of the high and medium dose group was significant different from the low dose group(P<0.05),and was extremely significant from the negative control(P<0.01).As for the hormone level,serum FSH,LH,E 2 levels tended to rise after taking medicine,P 4 tended to decline,with some reached extremely significant level at special time point(P<0.01).The high dose group had the most obvious changing trend,followed by the middle dose group and the serum reproductive hormones content had no significant difference among different time points in the negative control(P>0.05),with only a little increase of LH and E 2 on 16-19 days.The above results showed that Chinese medicine aphrodisiac powder could adjust reproductive hormone levels to achieve therapeutic purposes for cow’s persistent corpus luteum and the treatment effect of high dose group was the best.

关 键 词：奶牛 持久黄体 催情散 血清 生殖激素 

分 类 号：S857.21[农业科学—临床兽医学]