尿促性素的提取及其生物活性研究  

Research on Extraction of Menotropins and Their Biological Activities

作　　者：张夫永 李俊玲[1,2] 覃荣活[1,2] 贺芳 王世立[1,2] ZHANG Fuyong;LI Junling;QIN Ronghuo;HE Fang;WANG Shili(School of Medicine and Life Sciences,University of Jinan-Shandong Academy of Medical Sciences,Jinan 250022,China;Shandong Medicinal Biotechnology Centre,Jinan 250062,China)

机构地区：[1]济南大学-山东省医学科学院医学与生命科学学院,山东济南250022 [2]山东省医药生物技术研究中心,山东济南250062

出　　处：《济南大学学报（自然科学版）》2018年第3期199-203,211,共6页Journal of University of Jinan(Science and Technology)

基　　金：山东省医学科学院医药卫生科技创新工程

摘　　要：建立人绝经期促性腺激素(HMG)提取及初步纯化工艺,提取不同地区绝经期妇女尿液中HMG成分,进行免疫效价和生物活性测定,以苏木精-伊红染色法观察大鼠卵巢经HMG中间体注射后的改变,比较不同地区原尿提取的HMG中间体的质量;以高岭土吸附,二级丙酮提纯得到HMG中间体,采用酶联免疫吸附测定试剂盒测定卵泡刺激素(FSH)的免疫效价,生物测定法测定FSH生物活性。结果发现,采自临沂、济宁、济南、泰安、德州5地的样品的HMG中间体免疫效价分别为0.92、1.06、1.57、1.08、0.9 IU/mg,生物活性分别为1.02、0.94、1.21、0.77、0.76 IU/mg;经HMG中间体注射后,大鼠卵泡体积、质量均变大,且随剂量增加更明显;相比传统方法,本文中提取的HMG中间体活性有显著提高,采自济南地区的样品的HMG中间体生物活性最高,临沂、济宁两地的次之;同时,除临沂外,其他地区样品的HMG中间体生物活性均低于免疫效价。The method of extraction and preliminary purification of human memopausal gonadotropin(HMG)was established,HMG intermediates were extracted from urine of perimenopausal women in different regions,and the immunepotencies and biological activities of HMG intermediates were detected.The changes of rat ovaries after HMG intermediates injection were observed by hematoxylin-eosin staining.The quality of HMG intermediates extracted from original urine in different regions was compared with each other.HMG intermediates were obtained by the method of kaolin absorption and acetone two-step precipitation.The immunepotency of follicle-stimulating hormone was detected by enzyme-linked immunosorbent assay kit.The biological activity of FSH was detected by the follicle-stimulating hormone(FSH)bioassay.The results show that the FSH immunepotencies of HMG intermediates extracted from Linyi,Jining,Jinan,Taian,Dezhou are 0.92,1.06,1.57,1.08,0.9 IU/mg respectively,the FSH biological activities are 1.02,0.94,1.21,0.77,0.76 IU/mg,respectively.Rat follicles increase in volume and weight after HMG intermediates injection,and the trend is more obvious with the increase of dose.Compared with the traditional extraction methods,the quality of HMG intermediates in this study is significantly increased.The biological activities of HMG intermediates extracted from Jinan are the highest,and those of HMG intermediates extracted from Linyi and Jining are the next.The biological activities of all HMG intermediates are lower than their own immunepotencies except HMG intermediates extracted from Linyi.

关 键 词：人绝经期促性腺激素 卵泡刺激素 免疫效价 生物活性 

分 类 号：Q575.12[生物学—生物化学]