牛传染性鼻气管炎病毒LAMP检测方法的建立  

Development of LAMP Method for Infectious Bovine Rhinotracheitis Virus

作　　者：高峰[1] 王建峰 于伯华[1] 张丹[1] 刘向阳[1] 张琳[1] 秦志军[1] 唐泰山[3] Gao Feng;Wang Jianfeng;Yu Bohua;Zhang Dan;Liu Xiangyang;Zhang Lin;Qin Zhijun;Tang Taishan(Yancheng Exit-entry Inspection and Quarnatine Bureau,Yancheng,Jiangsu 224002,China;Ningbo Science and Technology Institute of Inspection and Quarantine,Ningbo,Zhejiang 315100,China;Jiangsu Exit-entry Inspection and Quarnatine Bureau,Nanjing,Jiangsu 210001,China)

机构地区：[1]盐城出入境检验检疫局,江苏盐城224002 [2]宁波检验检疫科学技术研究院,浙江宁波315100 [3]江苏出入境检验检疫局,江苏南京210001

出　　处：《中国动物检疫》2018年第5期87-90,共4页China Animal Health Inspection

基　　金：江苏检验检疫局科研项目(2016KJ14);宁波市科技创新团队(2015C110018)

摘　　要：为建立一种利用颜色判定的快速、简单、灵敏度高的检测方法,即可视化环介导等温扩增(LAMP)方法,采用针对牛传染性鼻气管炎病毒(IBRV)基因保守序列设计的特异引物,优化反应条件,建立了IBRV LAMP检测方法,并进行了特异性和灵敏性试验。结果显示,所建立的LAMP方法具有良好的特异性,其检出灵敏度为10~3 copies/μL,仅需1 h即可肉眼观察检测结果。结果表明,本试验建立的LAMP方法可用于IBRV的进出口检疫。In order to establish a rapid,simple and sensitive detection method which judges the result based on color change,a LAMP method was established for IBRV detection by designing specific primers according to gene sequences in conserved region and optimizing the reaction conditions,and specificity and sensitivity tests were also carried out.The results showed that the established LAMP method had good specificity,the detection threshold could reach 103 copies/μL,and the test result could be observed by naked eye after only 1 h.The results showed that the LAMP method established in this experiment could be applied to entry-exit inspection and quarantine of IBRV.

关 键 词：牛传染性鼻气管炎病毒 环介导等温扩增 检测 

分 类 号：S852.65[农业科学—基础兽医学]