大黄总蒽醌提取物对脑缺血再灌注损伤的保护作用及其机制  

Protective effect and mechanism of total anthraquinone extract of Rhei Radix et Rhizoma on cerebral ischemia-reperfusion injury

作　　者：万浩芳[1] 万海同[1] 周惠芬[1] 虞立[1] 陈烨[1] 胡锦祥[1] 石森林[1] WAN Hao-fang;WAN Hai-tong;ZHOU Hui-fen;YU Li;CHEN Ye;HU Jin-xiang;SHI Sen-lin(Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中成药》2018年第4期771-776,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81630105;81374053)

摘　　要：目的探讨大黄总蒽醌提取物抗脑缺血再灌注损伤的作用机制,为开发大黄总蒽醌类药物预防治疗脑缺血再灌注损伤提供参考依据。方法将雄性SD大鼠随机分为模型组、假手术组、尼莫地平组,大黄总蒽醌提取物高、中、低剂量组,每组8只,造模前一周开始灌胃给药,假手术组与模型组给予等体积生理盐水。末次给药30 min后,建立大鼠大脑中动脉局灶性栓塞(MCAO)模型,造模1.5 h再灌注24 h后,进行神经功能评分、脑指数、脑含水量、脑梗死体积的测定,使用ELISA试剂盒检测脑组织中超氧化歧化酶(SOD)活性,丙二醛(MDA)、一氧化氮(NO)及血清中白介素-6(IL-6)、白介素-1β(IL-1β)、肿瘤坏死因子(TNF)的含有量。结果大黄总蒽醌提取物能显著改善大鼠神经功能评分,降低脑指数、脑含水量,减小脑梗死面积(P<0.05),能显著提高脑组织中SOD活性(P<0.01),降低MDA、NO的水平(P<0.01),同时还可以显著降低血清中IL-6、TNF、IL-1β的含有量(P<0.01)。结论大黄总蒽醌提取物对大鼠脑缺血再灌注损伤有一定的保护及改善作用,其机制可能与抑制炎症反应和抗氧化相关。AIM To investigate the effect and mechanism of total anthraquinone extract of Rhei Radix et Rhizoma on cerebral ischemia-reperfusion injury and to provide relevant data references for its promising use in the management of cerebral ischemia-reperfusion injury.METHODS SD rats randomly assigned to model group,sham operation group,nimodipine group,total anthraquinone extract groups(high,medium and low dose),8 in each group,were orally administered with corresponding drugs daily for a week,with rats of the model group and sham operation group given the same volume of normal saline before the models established by middle cerebral artery occlusion(MCAO).MCAO started thirty minutes after final oral administration,and the induced ischemia went on for 1.5 h for a further reperfusion,24 h after which the neurological function score,brain index,brain water content and cerebral infarct volume were measured.Elisa kits were used to detect superoxide dismutase(SOD),Malondialdehyde(MDA),Nitric oxide(NO),interleukin-1 p(IL-l p),tumor necrosis factor(TNF),interleukin-6(IL-6).RESULTS The total anthraquinone extract of Rhei Radi%et Rhizoma significantly improved the neurological function score,decreased the brain index,brain water content,reduced the cerebral infarct volume(P<0.05),increased the activity of SOD in brain tissue(P<0.01),and reduced the levels of MDA and NO in brain tissue(P<0.01),and the levels of IL-6,TNF and IL-lp in serum(P<0.01)as well.CONCLUSION The obviously protective effect on rats,cerebral ischemia-reperfusion injury by total anthraquinone extract of Rhei Radix et Rhizoma may contribute to its inhibition of inflammatory response,and its existence as an antioxidant as well.

关 键 词：大黄 总蒽醌 炎症反应 抗氧化 脑缺血再灌注 

分 类 号：R285.5[医药卫生—中药学]