蛇尾草质量标准的研究  

Quality standard for Pogostemon auricularius

作　　者：王美琪[1] 王莉莉 马雯芳[1] 曾祥燕[1] 陈秋伶 覃美乐 WANG Mei-qi;WANG Li-li;MA Wen-fang;ZENG Xiang-yan;CHEN Qiu-ling;QIN Mei-le(Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学,广西南宁530001

出　　处：《中成药》2018年第4期857-861,共5页Chinese Traditional Patent Medicine

基　　金：广西科学技术厅项目(桂科重1598005-10);广西壮瑶药协同创新中心项目(桂教科研[2013]20);广西壮瑶药重点实验室项目(桂科基字[2014]32);广西重点学科(壮药学)(桂教科研[2013]16);广西八桂学者中药创新理论与药效研究(J13162);广西中医药大学大学生训练课题(2015DXS15)

摘　　要：目的建立蛇尾草Pogonostemon auricularius(L.)Hassk.的质量标准。方法对蛇尾草基源、性状、显微特征进行鉴别后,TLC法作定性鉴定,HPLC法测定毛蕊花糖苷含有量,《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含有量。结果 TLC斑点清晰,重复性好。毛蕊花糖苷在0.102~2.04μg范围内呈良好的线性关系(r=0.999 98),平均加样回收率97.99%,RSD 2.02%。14批样品中,水分、总灰分、酸不溶性灰分、浸出物含有量范围分别为6.47%~12.07%、7.59%~12.35%、0.23%~3.55%、13.28%~26.82%。结论该方法稳定可靠,可用于蛇尾草的质量控制。AIM To establisli the quality standard for Pofonosternof auriciularkus(L.)Hassk..METHODS The original,characteristic and microscopic features of P.auricularins were identified,after which TLC was used for qualitative identification,HPLC was adopted in the content determination of verl)ascoside,and the contents of water,total ash,acid-insoluble ash,extract were determined by Chinese Pharmacopoeia metiiods.RESULTS The clear TLC plots displayed good reproducibility.Verbascoside showed a good linear relationship within the range of 0.102-2.04"g(r=0.999 98)#whose average recovery was 97.99%witli the RSD of 2.02%.In four-teen batches of samples,thecontent ranges of water,total ash,acid-insoluble ash,extract were 6.47%-12.07%,7.59%-12.35%,0.23%-3.55%,13.28%-26.82%,respectively.CONCLUSION This sta-ble and reliable metliod can be used for the quality control of P.auricularius.

关 键 词：蛇尾草 毛蕊花糖苷 TLC HPLC 

分 类 号：R284.1[医药卫生—中药学]