替米沙坦对高脂喂养OLETF大鼠脂肪组织LPIN1表达的影响  

Effect of telmisartan on expression of LPIN1 in adipose tissue in High-fat-Diet-Fed OLETF Rats

作　　者：郑喜兰[1] 赵紫琴[2] 田凤石[3] 雒瑢[4] Zheng Xilan;Zhao Ziqing;Tian Fengshi;Luo Rong(Haihe Hospital of Tianjin,Tianjin 300350;Tianjin Hospital of Tianjin,Tianjin 300211;Fourth Central Hospital of Tianjin,Tianjin 300140;Tianjin Chest Hospital,Tianjin,300051)

机构地区：[1]天津市海河医院,天津300350 [2]天津市天津医院,天津300211 [3]天津市第四中心医院,天津300140 [4]天津市胸科医院,天津300051

出　　处：《天津药学》2018年第2期5-8,共4页Tianjin Pharmacy

摘　　要：目的:探讨替米沙坦对高脂喂养的OLETF大鼠脂肪组织中脂素基因(LPIN1)表达的影响及其部位差异性。方法:4周龄OLETF雄性大鼠30只,LETO大鼠12只作为正常对照(NC组)。8周龄开始OLETF大鼠饲以高脂饲料,22周龄口服葡萄糖耐量试验未发生临床糖尿病或糖耐量减退。将OLETF大鼠随机分为三组,高脂喂养OLETF大鼠组(OH组)10只、吡格列酮干预组(O-P组)10只和替米沙坦干预组(O-T组)10只。48周龄时,复查OGTT,计算稳态模型评估-胰岛素抵抗指数(HOMA-IR)。用ELISA方法测定血清生化指标,实时PCR检测皮下和内脏脂肪组织(SAT和VAT)中LPIN1的基因表达。结果:在内脏脂肪组织(VAT)中,O-P及O-T组与O-H组比较LIPIN1表达均降低(P<0.05),O-P组与O-T组无明显差异。在皮下脂肪组织(SAT)中O-T组与O-P组比较,有下降趋势但无统计学意义(P>0.05)。O-H组中LPIN1表达存在部位差异性(P<0.01)。O-T组LPIN1表达未见明显部位差异性。O-P组LPIN1表达在VAT低于SAT,但无统计学意义。结论:替米沙坦可能通过调节LPIN1表达部分激活PPARγ,促进OLETF大鼠内脏脂肪细胞的分化和成脂,改善代谢综合症的状态。Objective:To explore the regulation of telmisartan on the expression of LPIN1 in subcutaneous adipose tissue(SAT)and visceral adipose tissue(VAT)in high-fat diet fed OLETF rats and their tissue difference.Methods:Thirty four-week-old male OLETF rats were selected and 12 gender-and age-matched Long-Evans Tokushima Otsuka(LETO)rats were used as normal control.From 8 weeks of age.the OLETF rats were fed with high-fat diet.At 22 weeks of age,there was no case of impaired glucose tolerance or type 2 diabetes mellitus in OLETF rats tested by oral glucose tolerance test(OGTT).Then,these pre-diabetic OLETF rats were divided into telmisartan group(O-T group,5 mg/kg·d-1,n=10),pioglitazoue group(O-P group,10 mg/kg·d-1,n=8),and untreated control group(O-T group,n=10)according to the radom number table.LETO rats(n=12)were used as control group.OGTT was carried out at 48 weeks of age,and homeostasis model assessment of insulin resistance(HOMA-IR)was evaluated.The mRNA levels of LPIN1 in different fatty tissues were determined by real-time PCR.Results:In the VAT,the expression of LIPIN1 in O-P and O-T group was decreased compared with the O-H group(P<0.05),and there was no significant difference between group O-P and group O-T.In the SAT,O-T was compared with O-P,there was a decline trend but there was no statistical significance(P>0.05).The expression of LPIN1 in O-H was different(P<0.05;P<0.01)The expression of LPIN1 in O-T showed no obvious difference.The LPIN1 expression in O-P was lower than the SAT,but was not statistically significant.Conclusion:Telmisartan can promote human visceral fat cells differentiation into fat and improve the condition of metabolic syndrome,at least by partially regulate the expression of LPIN1.

关 键 词：米沙坦 代谢综合征 脂素基因 脂肪组织 过氧化物酶体增殖物活化受体Γ 

分 类 号：R965[医药卫生—药理学]