基于环介导等温扩增技术检测江苏省中部地区小麦赤霉病病菌的种群组成  

Detection of Fusarium spp. Causing Wheat Scab in the Middle Area of Jiangsu Using LAMP Assay

作　　者：许苗 冯慧 王淑琛 马家新 叶文武 郑小波[1] XU Miao;FENG Hui;WANG Shuchen;MA Jiaxin;YE Wenwu;ZHENG Xiaobo(College of Plant Protection/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)

机构地区：[1]南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,江苏南京210095

出　　处：《麦类作物学报》2018年第4期498-504,共7页Journal of Triticeae Crops

基　　金：科技部863计划项目(2012AA101501)

摘　　要：为明确江苏省中部地区小麦赤霉病病菌的种群组成,于2017年在江苏省镇江市、扬州市和南京市采集小麦赤霉病样本151份,采用可特异性识别亚细亚镰孢、禾谷镰孢、黄色镰孢、藤仓镰孢、燕麦镰孢、木贼镰孢和拟轮枝镰孢的环介导等温扩增(loop-mediated isothermal amplification,LAMP)检测技术检测了样本中该7种镰孢菌的分布情况。结果表明,从151份样本中共检测出7种镰孢菌中的6种。其中,亚细亚镰孢为优势种,检出率达100%;藤仓镰孢和禾谷镰孢为次优势种,检出率分别为10%和6%;黄色镰孢、燕麦镰孢和拟轮枝镰孢检出率依次为2.6%、2.0%和0.6%。小麦赤霉病存在复合侵染现象,从单份发病样本中最多检测到3种致病菌。LAMP法可用于小麦赤霉病病菌与种群组成的快速检测。To determine population structure and distribution of Fusarium species causing wheat scab(head blight)in the middle area of Jiangsu province,151 wheat samples showing symptoms of wheat scab from Zhenjiang,Yangzhou and Nanjing were collected.Seven Fusarium species causing wheat scab were detected using loop-mediated isothermal amplification(LAMP)assay,including F.asiaticum,F.graminearum,F.culmorum,F.fujikuroi,F.avenaceu m,F.equiseti,and F.verticillioides.Among 151 diseased wheat samples,six of these Fusarium species were detected by the LAMP assay.The results showed that the predominant causal pathogen of wheat scab was F.asiaticum,accounting for 100%of the total number of the tested samples.Detection ratio of F.fujikuroi and F.graminearum was 10%and 6%,respectively,and was second to that of F.asiaticum.Detection ratio of F.culmorum,F.avenaceu m,and F.verticillioides reached up to 2.6%,2.0%and 0.6%,respectively.Complex infection was found in samples of wheat scab,and at most three species complex were detected from one sample.A simple,rapid and novel technology for detecting Fusarium spp.causing wheat scab was developed in this study,and the population structure of Fusarium spp.causing the wheat disease in the middle area of Jiangsu was analyzed by the LAMP method.

关 键 词：小麦赤霉病 镰孢菌 种群 LAMP检测 

分 类 号：S512.1[农业科学—作物学] S435.1