基于核壳色谱柱快速测定栀子中栀子苷和西红花苷-1含量  

Rapid Determination of Geniposide and Crocin-1 in Gardeniae Fructus Based on Core Shell Chromatographic Column

作　　者：周小琴 卢瑞芯 司徒少金 汤丹 ZHOU Xiao-qin;LU Rui-xin;SITU Shao-jin;TANG Dan(Guangzhou Baiyunshan Mingxing Pharmaceutical Co.,Ltd.,Guangzhou 510250,China;College of Pharmacy,Jinan University,Guangzhou 510632,China)

机构地区：[1]广州白云山明兴制药有限公司,广东广州510250 [2]暨南大学药学院,广东广州510632

出　　处：《中国中医药信息杂志》2018年第5期86-89,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家科技重大专项(2011ZX09201-201-07)

摘　　要：目的建立快速同时测定栀子药材中栀子苷和西红花苷-1含量的分析方法。方法采用常规液相色谱仪系统,Agilent Poroshell 120 EC-C18核壳色谱柱(100 mm×4.6 mm,2.7μm),流动相为乙腈-0.2%甲酸水溶液,梯度洗脱,检测波长时间程序为240 nm(0~7 min)、440 nm(7~14 min)。采用液质联用技术对色谱图中的主要成分进行指认。结果栀子苷和西红花苷-1分别在0.2~6.0μg和0.04~1.2μg进样量范围内呈良好的线性关系(r=0.999 9);仪器精密度、稳定性、重复性试验RSD均小于3%;平均加样回收率分别为99.89%和101.74%,RSD均小于3%。采用液质联用技术对色谱图中的8个主要成分进行了指认,包括3个环烯醚萜类成分和5个西红花酸类成分。结论该方法简便、快速、准确、重复性好,可为建立高效的栀子药材质量评价标准提供依据。Objective To establish a rapid method for simultaneous determination of geniposide and crocin-1 in Gardeniae Fructus.Methods A conventional liquid chromatography system was used.An Agilent Poroshell 120 EC-C18 column(100 mm×4.6 mm,2.7μm)was used with the mixture of acetonitrile-0.2%formic acid solution as the mobile phase in gradient elution.Detection wavelength was as follow:240 nm(0–7 min),440 nm(7–14 min).At the same time HPLC-MS/MS technology was used to identify the main components of the chromatogram.Results The calibration curves of geniposide and crocin-1 were in good linearity among the ranges of 0.2–6.0μg and 0.04–1.2μg,respectively(r=0.999 9).For the precision test,stability test,repeatability test,the RSD were less than 3%.The average recoveries were 99.89%and 101.74%,respectively,and all the RSD were less than 3%.Eight major components were identified by HPLC-MS/MS technology,including three iridoids and five crocetin derivatives.Conclusion The developed method is simple,rapid,accurate and reproducible,which can provide a basis for quality evaluation of Gardeniae Fructus.

关 键 词：栀子 栀子苷 西红花苷-1 快速分析 

分 类 号：R284.1[医药卫生—中药学]