铅污染对不同生境芦苇体内抗氧化酶系统的影响  

The effects of Pb pollution on the antioxidant enzyme system of Phragmites australis(common reed) in different habits

作　　者：张娜[1,2,3] 杨双[4] 童非 朱阳春 范如芹[1] 卢信[1] 刘丽珠[1] 穆春生[3] 张振华[1] ZHANG Na;YANG Shuang;TONG Fei;ZHU Yang-chun;FAN Ru-qin;LU Xin;LIU Li-zhu;MU Chun-sheng;ZHANG Zhen-hua(Institute of Agricultural Resource and Environmental Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;Key Lab of Food Quality and Safety of Jiangsu Province-----State Key Laboratory Breeding B ase, Nanjing 210014,China;Key Laboratory of Vegetation Ecology ,Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun 130024,China;Grassland Station in Yanbian Korean Autonomous Prefecture , Dunhua 133700, China)

机构地区：[1]江苏省农业科学院农业资源与环境研究所,江苏南京210014 [2]江苏省农业科学院,省部共建国家重点实验室培育基地江苏省食品质量安全重点实验室,江苏南京210014 [3]东北师范大学草地科学研究所植被生态教育部重点实验室,吉林长春130024 [4]吉林省延边朝鲜族自治州敦化市草原站,吉林敦化133700

出　　处：《江苏农业学报》2018年第2期333-339,共7页Jiangsu Journal of Agricultural Sciences

基　　金：中国博士后科学基金面上项目(2017M621670);国家重点基础研究计划项目(2015CB150801);江苏省农业科技自主创新基金项目[CX(16)1051];东北师范大学植被生态教育部重点实验室开放课题(130028712);江苏省农科院基本科研业务专项资金项目[ZX(17)2017];江苏省农业科学院科研基金项目(6111637)

摘　　要：为评估不同生境芦苇对铅污染的耐受或抵抗能力,采用盆栽试验模拟淹水和干旱生境,探究铅污染对不同生境芦苇体内蛋白质、丙二醛含量以及抗氧化酶活性的影响。结果表明,随着铅处理浓度增加和胁迫时间增加,芦苇体内丙二醛含量显著增加,但过氧化氢酶、超氧化物歧化酶和过氧化物酶活性也均显著增强。相同浓度铅处理的干旱生境芦苇体内超氧化物歧化酶和过氧化物酶活性均小于淹水生境芦苇。胁迫60 d,铅处理的两种生境芦苇体内过氧化氢酶、超氧化物歧化酶和过氧化物酶活性均显著增强;胁迫90 d,铅处理的两种生境芦苇体内的超氧化物歧化酶和过氧化物酶活性均显著大于对照。说明水生芦苇具有较强的抗氧化能力,超氧化物歧化酶和过氧化物酶在抵抗铅诱导的氧化胁迫中发挥重要作用。To evaluate the tolerance or resistance of Phragmites australis(common reed)in different habits under Pb pollution,a pot experiment was conducted to explore the effect of Pb pollution on the contents of protein and malondialdehyde and activities of antioxidant enzymes(catalase,peroxidase and superoxide dismutase)in plants under simulating drowned and diy environment.The results showed that content of malondialdehyde and the activities of antioxidant enzymes in reeds increased along wdth increase of Pb concentration and its stress period.The activities of peroxidase and superoxide dismutase in reeds were less in the dry environment than those in drowned environment at the same level of Pb concentration.After60days of Pb stress,the activities of catalase,peroxidase and superoxide dismutase in reeds increased significantly in both water en\riron^nents.After90days of Pb stress,the activities of peroxidase and superoxide dismutase in reeds were signiticantly higher than those in control in both water env^iron^ments.These results indicated that the flooded reeds had a stronger antioxidant capacit^^by increasing the activities of antioxidant enz^^^mes(especially by peroxidase and superoxide dismutase).

关 键 词：芦苇 淹水 干旱 抗氧化酶 

分 类 号：X592[环境科学与工程—环境工程]