高效佐剂对猪细小病毒和圆环病毒二联灭活疫苗的免疫增强效果  

Effect of immunopotentiators on the efficacy of the bivalent vaccine of PPV and PCV2

作　　者：张雪花[1,2,3,4] 张道华 陆吉虎[1,2,3,4] 梅梅 华涛[1,2,3,4] 唐波 侯继波[1,2,3,4] 唐应华 ZHANG Xue-hua;ZHANG Dao-hua;LU Ji-hu;MEI Mei;HUA Tao;TANG Bo;HOU Ji-bo;TANG Ying-hua(Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China;Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014,China)

机构地区：[1]江苏省农业科学院动物免疫工程研究所,江苏南京210014 [2]江苏省农业科学院国家兽用生物制品工程技术研究中心,江苏南京210014 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [4]省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,江苏南京210014

出　　处：《江苏农业学报》2018年第2期361-367,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0500706);农业部公益性行业(农业)科研专项经费项目(201303046);江苏省农业科技自主创新基金项目[CX(15)1063]

摘　　要：为评价高效佐剂VA5对猪细小病毒和猪圆环病毒二联灭活疫苗的免疫增强效果,将VA5与猪细小病毒、猪圆环病毒疫苗混合免疫BALB/c小鼠和豚鼠。结果显示,VA5佐剂能明显提高猪细小病毒血凝抑制抗体效价和血清病毒中和抗体效价,亦能提高猪圆环病毒ELISA抗体和中和抗体效价,并可降低二种抗原的使用量。VA5佐剂显著提高小鼠和豚鼠免疫后的淋巴细胞刺激指数。攻毒后,VA5佐剂处理组动物脾脏PCV2/PPV病毒载量明显低于不含高效佐剂的免疫组。VA5佐剂能提高猪细小病毒、猪圆环病毒疫苗在小鼠/豚鼠中的抗体效价,提高细胞免疫力,降低攻毒后的排毒。To evaluate the effect of immunopotentiator VA5on the porcine par^^ovirus(P P V)and porcine circovirus type2(PCV2)bivalent inactivated vaccine(PPV-PCV2),BALB/c mice and guinea pigs were immunized the bivalent vaccine with or without VA5.The results showed that VA5significantly^improved the serum-virus neutralizing(S N)and hemagglutination inhibition antibo-dies titer against PPV,and similarly increased the ELISA antibody levels and SN antibody levels against PCV2.The VA5significantly reduced antigen contents in each dose of vaccine.Lymphocyte stimulation index of mice and guinea pigs were significantly enhanced in the treatment of bivalent vaccine plus VA5.After challenge with PCV2/PPV,the viral load of PCV2/PPV in the spleen of mice and guinea pigs were both significantly reduced in the group immunized with the bivalent vaccine in presence of VA5compared with that in the group immunized with bivalent vaccine absence of VA5.VA5adjuvant can improve antibody titer of the porcine par^^ovirus,porcine circovims vaccine in mice/guinea pig,improve cellular immunity and reduce the detoxification after attack.

关 键 词：高效佐剂 猪细小病毒 猪圆环病毒 抗体 细胞免疫 病毒载量 

分 类 号：S859.797[农业科学—临床兽医学]