H9N2亚型禽流感病毒对鸡的免疫抑制机制  

The immunosuppressive mechanism of H9N2 subtype avian influenza virus infection in chickens

作　　者：刘青涛[1] 李银[1] 刘娜[1,2] 杨婧[1] 韩凯凯[1] 刘宇卓[1] 赵冬敏[1] 黄欣梅[1] 田宇杰[1,3] LIU Qing-tao;LI Yin;LIU Na;YANG Jing;HAN Kai-kai;LIU Yu-zhuo;ZHAO Dong-min;HUANG Xin-mei;TIAN Yu-jie(Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Animal Disease Diagnostic and Immunology/Ministry of Agriculture,National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China;College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095,China;College of Animal Science, Guizhou University, Guiyang 550025,China)

机构地区：[1]江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心,江苏南京210014 [2]南京农业大学动物医学院,江苏南京210095 [3]贵州大学动物科学学院,贵州贵阳550025

出　　处：《江苏农业学报》2018年第2期368-373,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31502100);江苏省农业科技自主创新基金项目[CX(15)1058]

摘　　要：通过评价H9N2亚型禽流感病毒(AIVs)感染对于鸡新城疫和传染性支气管炎活疫苗免疫效力的影响,研究了H9N2 AIVs的免疫抑制机理。结果显示,H9N2 AIVs感染可降低鸡对鸡新城疫和传染性支气管炎活疫苗的抗体应答,但与对照组相比差异并不显著;而流式分析结果显示,与对照组相比H9N2 AIVs感染可显著降低鸡外周血中CD4+T淋巴细胞和CD8+T淋巴细胞的比率(P<0.05);抗原特异性的T淋巴细胞增殖试验结果显示,与对照组相比H9N2 AIVs感染鸡的外周血T淋巴细胞增殖活性显著下降(P<0.05);另外,H9N2 AIV感染还导致鸡血清中细胞因子IFN-γ和IL-4应答水平的下降,其中IFN-γ的应答水平与对照组相比差异显著(P<0.05)。说明H9N2 AIVs感染可抑制鸡的免疫应答,其中对细胞免疫应答的抑制更为明显。The influence of H9N2subtype avian influenza viruses(H9N2AIVs)infection on the immune efficacy of newcastle disease virus(N DV)and infectious bronchitis virus(IBV)vaccine was assessed to deter^nine the immune suppressive mechanism for H9N2virus.The results showed that the infection of H9N2virus reduced the antibody titers to the live vaccine of NDV and IBV,but this difference was not significant compared with the control group.However,the flow cytometric analysis of peripheral blood lymphocyte subpopulations showed that H9N2virus infection resulted in a significantly decrease in CD4+T lymphocyte and CD8+T lymphocyte proportion,and the antigen specific proliferation ability of peripheral blood T lymphocyte in H9N2virus infection group was also obviously suppressed compared with that of the control group.Moreover,the level of cytokines IFN-7and IL-4in serum of H9N2virus infection chickens was also lower than that of control group,and the difference of IFN-7was significant.The results suggested that H9N2AIVs infection could suppress the immune response to vaccine in chickens,and the cellular immune response was more significantly suppressed.

关 键 词：H9N2亚型禽流感病毒 鸡 免疫抑制 

分 类 号：S858.312.65[农业科学—临床兽医学]