不同特异种质罗汉果的遗传差异及杂交优势预测  

Genetic diversity of different Siraitia grosvenorii special germplasm and the prediction of heterosis

作　　者：覃信梅 蒋水元[2,3] 韩愈 向巧彦[2,3] 李虹 甘金佳[2,3] 梁勇诗 黄夕洋[2,3] QIN Xin-mei;JIANG Shui-yuan;HAN Yu;XIANG Qiao-yan;LI Hong;GAN Jin-jia;LIANG Yong-shi;HUANG Xi-yang(College of Life Science , Guangxi Normal University, Guilin 541004, China;Guangxi Institute of Botany , Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China;Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guilin 541006,China)

机构地区：[1]广西师范大学生命科学学院,广西桂林541004 [2]广西壮族自治区中国科学院广西植物研究所,广西桂林541006 [3]广西植物功能物质研究与利用重点实验室,广西桂林541006

出　　处：《江苏农业学报》2018年第2期425-431,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31660084);广西科学研究与技术开发计划项目(桂科能1598025-40;桂科重14124002-9);广西自然科学基金项目(2016GXNSFBA380164);广西植物功能物质研究与利用重点实验室资助项目(ZRJJ2016-11)

摘　　要：利用简单序列重复区间扩增多态性(ISSR)技术分析了罗汉果航天、无籽、野生、栽培4类种质的遗传差异,构建DNA指纹图谱和UPGMA聚类图,并进行杂交优势预测的应用研究。结果表明,筛选出的19个ISSR引物中的5个可直接鉴别罗汉果21份特异种质,种质间的遗传相似性系数为0.656~0.896,在相似性系数为0.795处的聚类结果分为5类。不同种质间存在一定的遗传变异,尤其是广西金秀(S17)、湖南永州(S18)和广西百色(S19)的野生种质与桂林主产区种质间,航天种质内以及雌雄株间都产生了较大的遗传差异。根据遗传差异的大小,对9个雌株预测了20个可能获得理想杂交优势的组合,推测雄株S13、S7和S9是较好的父本材料。该研究补充完善了罗汉果种质资源的遗传背景信息,从而为罗汉果种质的保护、创新和利用提供了理论基础及依据。The genetic diversity of four lines of Siraitia grosvenorii ger^nplasms(aerospace,seedless,natural and cultivated)was analyzed by inter-simple sequence repeat(I S S R),the DNA fingerprints and UPGMA dendrogram were established,and the heterosis was predicted.The results showed that five out of19selected ISSR primers could directly identify all21S.grosvenorii specific germplasms.Geneticsimilarity coefficients ranged from0.656to0.896,and the germplasms were divided into five groups at0.795.It revealed genetic diversity among different germplasms.Particularly,larger diversity existed among the germplasms from different geographic areas,i.e.Hunan Yongzhou(S18),Guangxi B a ise(S19),Guangxi Jin xiu(S17)and the major-producing area of Guilin,as well as within the aerospace germplasms,between male and female plants.Based on the genetic diversity,we deduced20parental combinations of nine female individuals,and aerospace S13,natural S7and S9were more excellent male parent.The research complemented new genetic background inlor^nation of S.grosvenorii and would provide the theoretical basis and reference for conser^^ation,innovation and utilization of these germplasms.

关 键 词：罗汉果 特异种质 遗传差异 简单序列重复区间扩增多态性(ISSR)指纹图谱 杂交预测 

分 类 号：S326[农业科学—作物遗传育种]