氧化还原酶介导生物级联催化研究进展  

Research Progress on Oxidoreductase Mediated Biocatalytic Cascades

作　　者：伍开琳 陈永正[1,2] WU Kai-lin;CHEN Yong-zheng(School of Pharmacy,Zunyi Medical University,Zunyi 563000,China;Green Pharmaceuticals Engineering Research Center of Guizhou Province,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医学院药学院,贵州遵义563000 [2]遵义医学院贵州省绿色制药工程研究中心,贵州遵义563000

出　　处：《合成化学》2018年第4期292-299,共8页Chinese Journal of Synthetic Chemistry

基　　金：国家自然科学基金资助项目(21162047;21562054;21262051)

摘　　要：氧化还原酶种类丰富,广泛应用于生物催化级联反应中。以细胞色素P450单加氧酶和羰基还原酶为例,综述了两者参与的级联催化反应及其在合成手性胺和手性醇等化合物中的应用,并对发展氧化还原酶级联催化反应面临的辅酶循环、限速步骤和化合物抑制等问题进行了讨论。Oxidoreductases contain various enzymes and are widely applied in biocatalytic cascade reactions.Oxidoreductase mediated biocatalytic cascades and its applications for synthesis of chiral amines and alcohols were sumed up,using cytochrome P450 monooxygenase and ketoreductase as examples.In addition,the problems such as cofactor regeneration,rate-limiting step and compound inhibition are discussed.

关 键 词：生物催化 级联反应 P450单加氧酶 羰基还原酶 综述 

分 类 号：O626[理学—有机化学]