17-AAG对人绒毛膜癌JAR细胞MMP-2和MMP-9表达的调控作用  

REGULATORY EFFECTS OF 17-AAG ON MMP-2 AND MMP-9 EXPRESSION IN HUMAN CHORIOCARCINOMA JAR CELLS

作　　者：谷苗 崔丽军 刘百艺 安国庆 许倩[1] GU Miao;CUI Li-jun;LIU Bai-yi(Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院,河北承德067000

出　　处：《承德医学院学报》2018年第3期186-189,共4页Journal of Chengde Medical University

基　　金：河北省高校重点学科建设项目资助(冀教高【2013】4号);河北省计生委项目(2013-A13);河北省教育厅项目(QN2016012)

摘　　要：目的:探讨热休克蛋白90(HSP90)抑制剂17-丙烯胺基-17-去甲氧基格尔德霉素(17-AAG)对人绒毛膜癌JAR细胞基质金属蛋白酶(MMP)-2、MMP-9蛋白表达的调控作用。方法:体外培养人绒毛膜癌JAR细胞,以不同浓度的17-AAG作用JAR细胞,采用MTT法检测JAR细胞的增殖抑制率,Western blotting法检测MMP-2、MMP-9蛋白的表达情况。结果:MTT结果显示,17-AAG对JAR细胞生长具有明显的抑制作用,并呈浓度依赖性。5μg/ml、10μg/ml、20μg/ml、40μg/ml的17-AAG作用后,JAR细胞MMP-2、MMP-9蛋白的表达明显下调。结论:17-AAG对人绒毛膜癌JAR细胞的增殖具有明显的抑制作用,且能抑制JAR细胞MMP-2、MMP-9蛋白的表达。Objective:To explore the effects of heat shock protein 90 inhibitor-17-AAG on matrix metalloproteinase-2(MMP-2)and MMP-9 expression in human choriocarcinoma JAR cells.Methods:The human choriocarcinoma JAR cells were cultured in vitro and treated with 17-AAG in different concentration.MTT was used to measure the proliferation inhibiting rate of JAR cells,Western blotting to detect the MMP-2 and MMP-9 expression.Results:MTT method showed that 17-AAG can obviously inhibit growth of JAR cells,and in concentration dependent manner.After the JAR cells were treated with 17-AAG in different concentration(5μg/ml,10μg/ml,20μg/ml,40μg/ml),the MMP-2 and MMP-9 expression in JAR cells down regulated obviously.Conclusions:17-AAG can significantly inhibit the proliferation of human choriocarcinoma JAR cells,and can inhibit the expression of MMP-2 and MMP-9 in JAR cells.

关 键 词：17-AAG 人绒毛膜癌JAR细胞 基质金属蛋白酶(2 MMP-2) 基质金属蛋白酶(9 MMP-9) 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]