杏扁PaCBF1基因的克隆及其生物信息学分析  

Cloning and Bioinformatic Analysis of PaCBF1 Gene in Prunus armeniaca L.

作　　者：王连荣[1] 薛拥志[2] 王宝地 WANG Lianrong;XUE Yongzhi;WANG Baodi(Horticulture Department,Hebei North University,Zhangjiakou 075131,China;College of Animal Science and Technology,Hebei North University,Zhangjiakou 075131,China;Science College,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院园艺系,河北张家口075131 [2]河北北方学院动物科技学院,河北张家口075131 [3]河北北方学院理学院,河北张家口075000

出　　处：《华北农学报》2018年第2期58-64,共7页Acta Agriculturae Boreali-Sinica

基　　金：河北省高等学校科学技术研究项目(NQ2015202);河北科技厅项目(17226303D);张家口科技局项目(1611047C)

摘　　要：CBF转录因子在多种植物的非生物逆境环境中起着重要的作用,可提高植物的抗逆性。为研究CBF基因在杏扁非生物逆境中的作用与功能,以杏扁品种围选1号为试验材料,利用RT-PCR技术克隆了1个CBF/DREB1转录因子基因CBF,命名为PaCBF1,Gen Bank登录号为MF491392。对其进行生物信息学分析,结果显示:该基因开放阅读框长度为723 bp,编码240个氨基酸,分子量为26.928 ku,等电点为6.35,亲水性平均值为-0.555;PaCBF1蛋白含有一个高度保守的AP2结构域,以及植物CBF蛋白的保守结构域PKKRAGRRVFKETRHP和DSAWR;二级结构预测显示该蛋白主要由38.75%的无规则卷曲、29.58%的α-螺旋、22.92%的延伸链和8.75%的β-转角组成;三级结构的预测结果显示含有α-螺旋、β-折叠和β-转角;该蛋白定位在细胞核,不存在信号肽序列,为非分泌性亲水蛋白,共含有13个磷酸化位点。结果为今后进一步深入研究杏扁CBF转录因子的功能以及对逆境环境的抵抗机制提供了理论基础。The C-repeat binding factors(CBF)/dehydration-responsive element-binding protein(DREB)are a group of conserved transcription factors that play an important role in the response and adaptation to environmental stress in many plants.In order to investigate the role of CBF transcription factors in plant abiotic stresses of Prunus armeniaca L.,a CBF/DREB1 transcription factor gene was cloned using reverse transcription-PCR(RT-PCR)from Weixuan No.1 and designated as PaCBF1.The GenBank accession number was MF491392.Bioinformatics analysis was carried out and the results showed that:The open reading frame(ORF)of PaCBF1 was 723 bp,which encoded a protein of 240 amino acids with a molecular weight of 26.928 ku and a pI of 6.35.The grand average of hydropathicity was-0.555.PaCBF1 protein contained an AP2 DNA-binding domain and two CBF signature sequences that one was PKKRAGRRVFKETRHP and the other was DSAWR.The prediction of secondary structure of PaCBF1 protein indicated that the encoded protein mainly contained 38.75%of random coil,29.58%ofα-helix,22.92%of extended strand and 8.75%ofβ-turn.Tertiary structure of PaCBF1 protein containedα-helix,β-pleated sheet andβ-turn.PaCBF1 protein was localized in the nucleus,it did not contain a signal peptide,and it was not a secreted protein.PaCBF1 protein contained 13 phosphorylation sites.These results provided a theoretical basis for the further study of the function of the kernel apricot CBF transcription factor and the resistance mechanism to the stress environment.

关 键 词：杏扁 PaCBF1 基因克隆 生物信息学 

分 类 号：Q78[生物学—分子生物学] S662.03[农业科学—果树学]