晚清书画裱件一株霉菌的鉴定及培养条件优化  

Identification and Culture Conditions Optimization of Mold from the Late Qing Dynasty Calligraphy and Painting

作　　者：李其久[1] 段大程[1] 刘辰澍 李梓铭 仲雨微 张可心[4] 孙超 陈长兰[5] 贲松彬[1] LI Qi-jiu;DUAN Da-cheng;LIU Chen-shu;LI Zi-ming;ZHONG Yu-wei;ZHANG Ke-xin;SUN Chao;CHEN Chang-lan;BEN Song-bin(Liaoning University of Life Sciences,Shenyang 110036,China;Liaoning Province Shiyan High School,Shenyang 110841,China;Shenyang eleventh middle school,Shenyang 110034,China;Liaoning University of Archives Center,Shenyang 110036,China;Liaoning University of Pharmacy,Shenyang 110036,China)

机构地区：[1]辽宁大学生命科学院,辽宁沈阳110036 [2]辽宁省实验中学,辽宁沈阳110841 [3]沈阳市第十一中学,辽宁沈阳110034 [4]辽宁大学档案中心,辽宁沈阳110036 [5]辽宁大学药学院,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2018年第1期59-63,共5页Journal of Liaoning University：Natural Sciences Edition

基　　金：国家自然科学基金项目(31371085);辽宁省科学事业公益研究基金项目(2016003007);辽宁省档案局科技项目计划(L-2017-B-1)

摘　　要：对晚清书画裱件菌斑菌株复苏分离鉴定及培养条件优化.以改良马铃薯葡萄糖琼脂培养基、萨氏培养基和查氏培养基作为复苏培养基.采用三点植入法和插片法对菌株形态学观察,通过PCR扩增、测序、构建系统发育树对菌株进行分子生物学鉴定.结果成功复苏一株霉菌,菌株鉴定为篮状菌属,最佳培养条件为改良后PDA培养基,培养温度30℃,pH 6.0,震荡频率130 r/min.We revived,isolated,identified,and optimized the recovery of the mold on the late Qing Dynasty painting and calligraphy.The modified potato dextrose agar culture and SA culture medium serve as the reviving culture medium.The morphological observation was carried out by three point method and insert method;the molecular biological identification was carried out by PCR amplification,sequencing and the establishment of the phylogenetic tree.A strain of fungus was successfully recovered in the experiment.The optimum cultivation culture is the PDA culture medium,temperature 30℃,oscillation frequency 130 r/min,pH6.0.

关 键 词：纸质文物 霉菌 鉴定 

分 类 号：Q93.331[生物学—微生物学]