红芪总黄酮对人白血病细胞诱导分化的影响分析  

Analysis of the Influence of Flavonoids on the Induction Differentiation of Human Leukemia Cells

作　　者：邓婉蓉[1] Deng Wan-rong(Gansu Traditional Chinese Medicine University,Gansu Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000

出　　处：《临床医药文献电子杂志》2018年第14期9-10,共2页Electronic Journal of Clinical Medical Literature

摘　　要：目的分析红芪总黄酮对人白血病细胞诱导分化影响。方法使用红芪总黄酮对HL-60细胞进行干预,用流式检测细胞周期及凋亡、用荧光PCR技术检测CD11b以及C-fos表达。结果:HL-60细胞使用浓度为60,80以及100 mg/L的红芪总黄酮干预后,和对照组相比,干预组细胞功能、表面标记检测明显上升。P<0.05.使用不同浓度的红芪总黄酮干预后,HL-60细胞周期停滞在G0/G1以及G2/M期。存在量效依赖关系,与之相对应的S期细胞减少,但促细胞凋亡作用不显著。与对照组相比,干预组相关数据存在统计学差异(P<0.05).处理组之间的C-fos表达呈现出剂量依赖关系。结论红芪总黄酮促进HL-60细胞机制可能为调控增殖分化有关基因表达,减少DNA合成以及转录,降低S期间细胞数量,加长细胞倍增时间,继而实现抑制细胞生长,诱导细胞分化作用。Objective To analyze the effect of flavonoids on human leukemia cell induced differentiation.Methods The HL-60 cells were interfered with HL-60 cells using the red stilbene flavone,and the expression of CD11b and C-fos was detected by the flow test cell cycle and apoptosis,and the fluorescence PCR technique was used to detect CD11b and c-fos.Results After the HL-60 cell concentration was 60,80 and 100 mg/L of the total flavonoid intervention,the intervention group cell function and surface marker detection increased significantly compared with the control group(P<0.05).The HL-60 cell cycle was stagnated at G0/G1 and G2/M period after the use of the different concentrations of the flavonoids.There was a quantitative dependence relationship,and the corresponding S phase cell decreased,but the effect of apoptosis was not significant.Compared with the control group,the correlation data of the intervention group was statistically different,and the c-fos expression in the treatment group showed a dose-dependent relationship.Conclusion:Red stilbene flavonoids-HL 60 cells mechanism for regulating the proliferation differentiation related gene expression,reduce the DNA synthesis and transcription,reduce S during cell number,elongated cells doubling time,and then realize the inhibition of cell growth and induce cell differentiation.

关 键 词：红芪 总黄酮化合物 C-FOS基因 HL-60细胞 

分 类 号：R285.5[医药卫生—中药学]