鸢尾丝囊霉(Aphanomyces iridis)引起的烟草漂浮苗根腐病的初步报道  

Preliminary report on tobacco seedling root rot induced by Aphanomyces iridis in floating bed

作　　者：李小杰[1] 李淑君[1] 李成军[1] 胡亚静[1] 康业斌[2] 邱睿[1] 陈奇园 白静科 陈玉国[1] LI Xiaojie;LI Shujun;LI Chengjun;HU Yajing;KANG Yebin;QIU Rui;CHEN Qiyuan;BAI Jingke;CHEN Yuguo(Key Laboratory for Green Preservation&Control of Tobacco Diseases and Pests in Huanghuai Growing Area/Tobacco Research Institute of Henan Academy of Agricultural Sciences,Xuchang 461000,Henan,China;College of Forestry,Henan University of Science and Technology,Luoyang 471000,Henan,China)

机构地区：[1]烟草行业黄淮烟区烟草病虫害绿色防控重点实验室河南省农业科学院烟草研究所 [2]河南科技大学林学院,河南省洛阳市天津路70号471000

出　　处：《中国烟草学报》2018年第2期125-128,共4页Acta Tabacaria Sinica

基　　金：中国烟草总公司重点项目"河南烟草病虫害绿色防控关键技术研究与应用"(201741000027063);河南省烟草公司科技项目"河南省烟草主要病虫害预测预报及综合防治示范"(HYKJ201608);河南省财政预算项目"烟草根茎类病害拮抗细菌的筛选及作用机理研究"(豫财预【2017】76-4);河南省农科院自主创新项目"烟草真菌类病害的采集;分离及快速检测体系的建立"(2017ZC21)

摘　　要：为明确近年来烟草漂浮育苗过程中出现的疑似根腐病的病原,2017年5月分别从河南省宝丰、泌阳、许昌、临颍等烟区苗床共采集12个具有代表性的病害样本,进行病原菌分离、形态学鉴定、18S rDNA-ITS序列分析和致病性测定。结果表明,从样本中分离纯化出10个真菌菌株,其18S rDNA-ITS序列与NCBI数据库中鸢尾丝囊霉(Aphanomyces iridis)序列同源性均为99%。漂浮苗及盆栽接种试验结果表明,烟苗接种后均有不同程度的发病,发病率为100%。将发病组织在PDA培养基上进行分离培养,也同样得到丝囊霉菌。本研究根据发病症状和病原菌,首次明确了鸢尾丝囊霉可引起烟草漂浮苗根腐病。To ascertain root rot pathogen,12 samples were collected from Baofeng,Biyang,Xuchang,Linying of Henan province in May 2017,and were analyzed by pathogenicity test.Morphological characteristics were identified and 18S rDNA-ITS sequence analysis was conducted.A total of 10 fungal isolates were obtained from these samples,and their 18S rDNA-ITS sequences were 99%homologous to Aphanomyces iridis sequence in the NCBI database.Inoculation experiment of floating and potted plants showed that the incidence was 100%,and the same pathogen was also separated from diseased tissue on PDA medium.According to symptom and pathogen,the disease was identified as tobacco seedling root rot in floating bed which was caused by Aphanomyces iridis.This is the first time that Aphanomyces proved to have caused tobacco seedling root rot.

关 键 词：烟草漂浮育苗 根腐病 病原鉴定 丝囊霉属 致病性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]