模拟霜冻条件对黄瓜幼苗生长及抗氧化酶活性的影响  

Effects of simulated frost condition on growth and antioxidant enzyme activity of cucumber seedlings

作　　者：赵曾菁 宋奇琦 赵虎[1] 吴星[1] 王萌[1] 李衍素[3] 闫妍[3] 覃挺 龚明霞[1] 何志[1] 王日升[1] ZHAO Zeng-jing;SONG Qi-qi;ZHAO Hu;WU Xing;WANG Meng;LI Yan-su;YAN Yan;QIN Ting;GONG Ming-xia;HE Zhi;WANG Ri-sheng(Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;College of Agriculture,Guangxi University,Nanning 530004,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hengxian 668 Greenhouse Vegetables Planting Professional Cooperation,Hengxian,Guangxi 530300,China)

机构地区：[1]广西农业科学院蔬菜研究所,南宁530007 [2]广西大学农学院,南宁530004 [3]中国农业科学院蔬菜花卉研究所,北京100081 [4]横县六六八大棚蔬菜种植专业合作社,广西横县530300

出　　处：《南方农业学报》2018年第4期757-762,共6页Journal of Southern Agriculture

基　　金：广西科技计划项目(桂科AD17129042;桂科AB16380065);广西农业科学院科技发展基金项目(桂农科2017JM48);南宁市科学研究与技术开发计划项目(20162327)

摘　　要：【目的】分析防霜冻技术对模拟霜冻过程中黄瓜幼苗形态、抗氧化酶活性和细胞膜透性的影响,为黄瓜防霜冻技术推广应用提供技术支持。【方法】在模拟自然霜冻条件下对黄瓜幼苗进行防霜冻技术处理(TR),在降温处理后不同时期调查黄瓜幼苗的株高、茎粗、叶长和叶宽及统计冷害指数,并在温度降至10℃/5℃前1 d(-1 d)及下降至10℃/5℃后1、3和5 d测定其抗氧化酶活性等生理指标。【结果】在模拟自然霜冻条件下,降温处理后3 d TR组黄瓜幼苗的相对株高为106.00%,显著高于对照组(CK,95.50%)(P<0.05,下同),相对茎粗、相对叶长和相对叶宽与CK组无显著差异(P>0.05,下同);降温处理后5 d TR组的冷害指数为40.67%,显著低于CK组。降温处理-1 d,TR组黄瓜幼苗的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著高于CK组,降温处理后1~3 d,SOD和CAT活性呈上升趋势,且高于CK组,但差异不显著;过氧化物酶(POD)活性在降温处理的-1~2 d高于CK组,在降温处理后3~5 d低于CK组,但差异均不显著;TR组与CK组的过氧化氢(H_2O_2)含量在降温处理的-1~5 d均呈上升趋势,TR组的H_2O_2含量低于CK组;TR组的丙二醛(MDA)含量在降温处理前后变化不明显,但在降温处理后1和5 d显著低于CK组。【结论】在霜冻来临前对黄瓜幼苗进行防霜冻技术处理,能有效提高其对霜冻的耐受性,显著减轻黄瓜冷害程度。【Objective】To provide technical support for the application and popularization of frost-prevention for cucumber,the effects of frost-prevention technique on morphology,antioxidant enzyme activity and cell membrane permeability of cucumber seedlings were analyzed under simulated frost condition.【Method】Cucumber seedlings were treated with frost-prevention technique(TR)under simulated frost condition,and then plant height,stem diameter,leaf length,leaf width and index of chilling injury were investigated at different times after temperature drop treatment.Antioxidant enzyme activity and other physiological indexes were measured 1 d before temperature dropped to 10℃/5℃(-1 d)and 1,3,5 d after temperature dropped to 10℃/5℃.【Result】Under simulated natural frost conditions,the relative plant height of cucumber seedlings after 3 d of TR group was 106.00%,which was significantly higher than that of CK 95.50%(P<0.05,the same below).There was no significant difference in relative stem diameter,relative leaf length,and relative leaf between TR and CK(P>0.05,the same below).The chilling injury indexes of TR group was 40.67%after 5 d temperature drop treatment,which was significantly lower than that of CK.Superoxide dismutase(SOD)and catalase(CAT)activities of cucumber seedlings were significantly higher than those of CK after TR group for-1 d.The activities of SOD and CAT showed an upward trend after 1-3 d of treatment and were higher than those of CK,but the difference was not significant.Peroxidase(POD)activity of TR during-1-2 d of treatment was higher than CK,and it was lower than CK 3-5 d after treatment,but the difference was not significant.The content of hydrogen peroxide(H2O2)in TR group and CK group increased during-1-5 d of temperature drop treatment.The content of H2O2 was lower than that of CK after treatment.Malondialdehyde contents(MDA)of TR group did not change greatly before and after temperature drop treatment,but it was significantly lower than that of CK on day 1 and day 5 after treatme

关 键 词：黄瓜 模拟霜冻 防霜冻技术 抗氧化酶活性 丙二醛(MDA)含量 

分 类 号：S642.2[农业科学—蔬菜学]