广西现行家蚕品种两广二号原种随时孵化技术  

Instantaneous hatching technique for silkworm variety Liangguang 2 stock in Guangxi

作　　者：李燕飞 李乙 蒋玉莲 韦红群 何珊珊 莫云霞 LI Yan-fei;LI Yi;JIANG Yu-lian;WEI Hong-qun;HE Shan-shan;MO Yun-xia(Guangxi Academy of Sericulture Science,Nanning 530007,China)

机构地区：[1]广西蚕业科学研究院,南宁530007

出　　处：《南方农业学报》2018年第4期787-793,共7页Journal of Southern Agriculture

基　　金：国家桑蚕产业技术体系建设项目(CARS-22-SYZ19)

摘　　要：【目的】明确保护时间、冷藏时间和浸酸时间3个因素对两广二号原种短期冷藏浸酸及延期冷藏浸酸实用孵化率的影响,为调节蚕种供应期及广西家蚕原种短期冷藏浸酸处理过程中制定不同冷藏时间的蚕种入库和盐酸刺激量标准提供依据,也为改进当前的短期冷藏浸酸技术提供指导。【方法】以两广二号原种4个亲本(芙蓉×932、932×芙蓉、湘晖×7532和7532×湘晖)为试验材料,按入库前保护时间、冷藏时间及浸酸时间3个因素3水平设计正交试验,调查4个亲本各因素不同水平的实用孵化率,筛选提高实用孵化率的最佳组合,并在此基础上选取孵化效果较好的亲本探讨延期冷藏适期。【结果】通过正交试验分析,4个亲本在不同冷藏天数中均可找到各因素条件较好的最高实用孵化率处理组合,其中,芙蓉×932为48 h-30 d-6 min,932×芙蓉为48 h-30 d-5 min,湘晖×7532为48 h-30 d-7 min,7532×湘晖为64 h-40 d-6 min。短期冷藏孵化效果较好的两个亲本(芙蓉×932和湘晖×7532)延期冷藏试验区与对照区的实用孵化差异不显著(F<F_(0.05),下同),两个亲本试验区和对照区蚁蚕的绝食经过时数同为120 h,同一时间内试验区和对照区蚁蚕死亡率差异也不显著。【结论】保护时间、冷藏时间、浸酸时间3个因素均对蚕种实用孵化率有一定影响,在实际生产中,蚕种提早出库首先要选择耐酸力强的品种,其次要根据入库时的卵龄即滞育程度确定盐酸刺激强度,最后在入库冷藏时间到期后分批取蚕卵解剖,了解蚕种的滞育进程。同时根据生产成本、劳动条件等实际情况,适当调整冷藏浸酸标准,即可获得最符合实际生产的理想实用孵化率。【Objective】The effects of three factors including protection time,refrigeration time and acid soaking time on the practical hatching rate of short-term refrigeration and acid treatment,and deferred short-term refrigeration and acid treatment for Liangguang 2 stock were studied to provide references for the adjustment of silkworm egg supply period,the standard formulation on the storage of silkworm eggs and acid stimulus dose in the process of short-term refrigeration and acid treatment of silkworm stock in Guangxi,as well as the improvement of existing short-term refrigeration and acid soaking treatment technology.【Method】Four parents of Liangguang 2 stock(Furong×932,932×Furong,Xianghui×7532,7532×Xianghui)were used as the test materials.Orthogonal test with three levels of three factors including protection time,refrigeration time and acid soaking time was designed to investigate the practical hatching rate of four parents at different levels of factors.The purpose was to find the best combination for increasing hatching rate,and on this basis,parents with fine hatching results were selected to study suitable period of deferred refrigeration.【Result】Through the analysis of orthogonal test,treatment combination of the highest practical incubation rate with desirable factors can be found in different refrigerated days for the four parents.In which,Furong×932,932×Furong,Xianghui×7532 and 7532×Xianghui were 48 h-30 d-6 min,48 h-30 d-5 min,48 h-30 d-7 min and 64 h-40 d-6 min respectively.There was not significant difference in practical hatchability between experimental plot and control plot for deferred refrigeration of two parents(Furong×932 and Xianghui×7532)which had sound hatching effects in short-term refrigiration(F<F0.05,the same below),the fasting survival hours of newly-hatched larva in experimental plot and control plot were both 120 h,meanwhile the difference in mortality of newly-hatched larva between experimental plot and control plot was not significant.【Conclusion】Three factor

关 键 词：家蚕 两广二号 短期冷藏 浸酸 实用孵化率 

分 类 号：S882.35[农业科学—特种经济动物饲养]