小鼠耳蜗血管纹血-迷路屏障免疫组织化学研究  

An Immunohistochemical Study of Cochlear Blood-labyrinth Barrier in Mice

作　　者：塞娜[1] 张桐[1] 吴军[1] 韩维举[1] SAI Na;ZHANG Tong;WU Jun;HAN Weiju(Department of Otolaryngology Head and Neck Surgery,Chinese PLA General Hospital,Beijing,100853,China)

机构地区：[1]解放军总医院耳鼻咽喉头颈外科,北京100853

出　　处：《中华耳科学杂志》2018年第2期205-211,共7页Chinese Journal of Otology

基　　金：国家自然科学基金面上项目(No:81770992;81470683)~~

摘　　要：目的利用免疫组织化学、免疫荧光染色及激光共聚焦显微镜技术研究小鼠血管纹组织形态、血-迷路屏障的组成,为耳蜗血管纹血-迷路屏障病理生理学研究提供基础。方法快速分离出完整的小鼠血管纹组织,应用免疫组织化学、免疫荧光染色等方法对血管纹上各层组织、细胞进行标记,利用激光共聚焦显微镜详细观察其形态结构特点,观察血-迷路屏障的组成及形态特点。结果完整解剖出小鼠的血管纹组织呈自然弯曲状态,颜色为半透明淡红色,免疫荧光染色观察三层细胞,边缘细胞呈近圆形的多边形,致密排列,其紧密连接蛋白呈线性分布;基底细胞呈扁长形连成一片;毛细血管网夹于边缘细胞与基底细胞之间贯穿血管纹全程,周细胞伴行血管并包裹毛细血管;中间细胞的血管周巨噬细胞呈树枝样,其足突伸出与微血管壁紧密接触。结论边缘细胞、中间细胞、基底细胞及毛细血管网构成耳蜗血管纹的基本结构,毛细血管内皮细胞、基底膜、周细胞、血管周巨噬细胞通过细胞间的紧密连接共同组成血-迷路屏障。Objective To study morphology of the Stria vascularis and composition of the blood-labyrinth barrier(BLB)in mice by immunohistochemistry,immunofluorescence staining and confocal laser scanning microscopy,to facilitate the study of its pathophysiology.Methods Intact stria vascularis tissue was rapidly isolated,and then labeled by immunohistochemistry and immunofluorescence.Morphological and structural characteristics were observed by laser confocal microscopy.Results The intact stria vascularis in mice was naturally curved and translucent reddish in color.Three layers of cells were observed by immunofluorescence staining.The marginal cell was essentially hexagon in shape with tight junction proteins linearly distributed.The basal cells were elongated and flat.The stria capillary network was sandwiched between the marginal and basal cells,accompanied and wrapped by pericytes.Perivascular-resident macrophages in the intermediate cells were dendritic,with their processes in close contact with microvessels.Conclusion Marginal cells,intermediate cells,basal cells and the capillary network constitute the basic structures of the cochlear stria vascularis.Endothelial cells,basilar membrane,pericytes and Perivascular-resident macrophages form a blood-to-labyrinth barrier through tight junctions between cells.

关 键 词：血管纹 血-迷路屏障 血管周巨噬细胞 周细胞 形态学 

分 类 号：R764[医药卫生—耳鼻咽喉科]