TRPC6、α-actinin-4在阿霉素肾病大鼠肾组织的表达差异及意义  

Expression and significance of TRPC6 and alpha-actinin-4 in renal tissue of rats with adriamycin nephropathy

作　　者：杜宇[1] 史应进[1] 张艳辉[1] 贾妮亚[1] 范俊英[1] 王彩丽[1] DU Yu;SHI Ying-jin;ZHANG Yan-hui;JIA Ni-ya;FAN Jun-ying(The First Affiliated Hospital of Baotou Medical College,Inner Mongolia Baotou 014010,China)

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010

出　　处：《临床医药文献电子杂志》2017年第77期15061-15063,共3页Electronic Journal of Clinical Medical Literature

基　　金：内蒙古自治区高等学校科学研究项目(批准号:NJZY14267)

摘　　要：目的分析TRPC6、α-actinin-4在大鼠正常肾组织和阿霉素肾病大鼠不同病理类型肾组织的表达差异,并以此讨论两个分子在蛋白尿产生中相互的关系以及可能发生的作用。方法采用阿霉素大鼠肾病模型,分析TRPC6、α-actinin-4在不同病理类型大鼠肾组织中的表达。结果 (1)TRPC6在微小病变和局灶节段性肾小球硬化肾小球内的的表达水平较对照组显著增多(P<0.05),且在局灶节段性肾小球硬化组中增多较微小病变组更加显著(P<0.05)。(2)α-actinin-4在微小病变组表达增加(P<0.05),而在局灶节段性肾小球硬化组表达与对照组无明显差异。(3)随着TRPC6的表达的增加α-actinin-4的表达下调(P<0.01)。结论 (1)TRPC6的表达在不同病理类型间存在差异,其表达的增强促进肾小球硬化。(2)α-actinin-4在不同病理类型间存在差异,其表达的变化间接反映足细胞的损伤,提示了病变的活动情况。(3)随着TRPC6表达的增加造成足细胞损伤、脱落、肾小球硬化,导致α-actinin-4的表达下调。Objective The different gene expression of TRPC6、α-actinin-4 in between normal kidneys tissues and abnormal kidneys tissues of the dissimilar pathological types was researched in this study,in order to investigated the interaction and co-relationship of these molecules and the progress of nephrotic-proteinuria.Methods Preparation by adriamycin rat model,analysis of TRPC6,alpha actinin-4 expression in renal tissue in rats in different pathological type.Results(1)The expression of TRPC6 in MCNR-group and FSGR-group was significantly higher than Control-group(P<0.05),and this expression in FSGR-group was also significantly higher than MCNR-group(P<0.05).(2)The expression ofα-actinin-4 in MCNR-group and FSGR-group was significantly higher than Control-group(P<0.05),while there was no difference in MCNR-group and FSGR-group(P>0.05).3.The expression ofα-actinin-4 was to descend as long as the one of TRPC6(P<0.05).Conclusion(1)The expression of TRPC6 was in difference among the dissimilar pathological types of nephrosis,and the augmented expression of TRPC6 could aggravate glomerulosclerosis.(2)There may be some co-relationship between the expression ofα-actinin-4 and the damage of foot cells.(3)The damage and abscission of foot cells and the glomerulosclerosis were to get worse as long as the raised expression of TRPC6.In result,the expression ofα-actinin-4 was to descend.

关 键 词：TRPC6 Α-ACTININ-4 大鼠 表达 

分 类 号：R285.5[医药卫生—中药学]