切花小菊主要分枝性状的混合遗传分析  

Mixed inheritance analysis of branching traits in spray cut chrysanthemum

作　　者：杨信程 苏江硕 吴洋洋[1] 张飞[1] 管志勇[1] 陈发棣[1] 房伟民[1] YANG Xincheng;SU Jiangshuo;WU Yangyang;ZHANG Fei;GUAN Zhiyong;CHEN Fadi;FANG Weimin(College of Horticulture/Key Laboratory of Landscape Agriculture,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院/农业部景观农业重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2018年第3期440-446,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31372092);上海市种业发展项目[沪农科种字(2016)第1-14号]

摘　　要：[目的]本文旨在解析切花小菊一、二级分枝性状的变异情况和主基因遗传效应。[方法]以切花小菊‘南农雪峰’בMonalisa’F_1代为试验材料,调查了F_1分离群体的总侧芽数、中上部一级分枝数、一级分枝长度、一级分枝角度、二级分枝总数、二级分枝长度、二级分枝角度在2015年和2016年的表型变异,利用F_2群体主基因+多基因混合遗传模型方法分析了这7个分枝性状。[结果]7个分枝性状均呈连续分布,变异系数为12.89%~61.55%;各分枝性状均存在正向或负向的中亲优势和超亲分离个体,其中总侧芽数、中上部一级分枝数、一级分枝角度和二级分枝总数的中亲优势较大,中亲优势率分别为-9.69%、-9.10%、12.99%和25.51%。混合遗传分析表明,总侧芽数、二级分枝长度、一级分枝角度和二级分枝角度符合0MG模型,无主基因控制;中上部一级分枝数和二级分枝总数符合2MG-AD模型,由2对加-显性主基因控制;一级分枝长度符合1MG-AD遗传模型,由1对加性-显性主基因控制。中上部一级分枝数、一级分枝长度、二级分枝总数的主基因遗传率分别为74%、46%、72%。[结论]初步明确了切花小菊主要分枝性状的遗传变异和主基因遗传效应,对今后切花小菊的株型育种具有重要参考价值。[Objectives]The present study is to investigate the genetic variability and major gene effect of the primary and secondary branch traits in spray cut chrysanthemum.[Methods]Seven branching traits,i.e.,total lateral bud number,up branch number,primary branch length,primary branch angle,total secondary branch number,secondary branch length,and secondary branch angle of the segregating‘Nannongxuefeng’בMonalisa’F 1 population were measured in 2015 and 2016,and the major gene effects for each branch trait were analyzed using mixed major gene plus polygene inheritance model in F 2 population.[Results]All the investigated branch traits showed continuous variations,with the coefficient of variation ranging from 12.89%to 61.55%.The phenoma of mid-paraent heterosis and extra-parent individuals existed generally for every branching trait,but transgressive heterosis was not yet to take shape in the F 1population,and the mid-paraent heterosis was relatively large for total lateral bud number,up-part primary branch number,primer branch angle and total secondary branch number,with the ratio of mid-parent heterosis calculated at-9.69%,-9.10%,12.99%and 25.51%,respectively.The mixed inheritance analysis indicated that the inheritances of total lateral bud number,secondary branch length,primary branch angle and secondary branch angle fit to the 0MG model with no major genes;the up primary branch number and total secondary branch number fit to the 2MG-AD model,governed by two pairs of major genes showing additive-dominant effect;the secondary branch length accords with the 1MG-AD model,controlled by a pair of major gene displaying additivity and part dominance or super-domimance effect.The heritability of major genes for the up-part primary branch number,primary branch length,total secondary branch number and secondary branch length were calculated at 74%,46%and 72%,respectively.[Conclusions]The findings present an insight into the genetic variability and major gene effects of important branching traits of spray cut chrysa

关 键 词：切花小菊 分枝性 主基因+多基因混合遗传模型 遗传分析 

分 类 号：S682.11[农业科学—观赏园艺] Q348[农业科学—园艺学]