6-姜酚对丙酮醛诱导的HK-2肾小管上皮细胞损伤的保护作用  

6-gingerol protects HK-2 cells against injury induced by methylglyoxal

作　　者：陈燕玲[1] 罗婷 吴秀香[1] 凡栋 李海洲 曾洪敏 康毅 王晓玲[1] 黄霜枝 CHEN Yan-ling;LUO Ting;WU Xiu-xiang;FAN dong;LI Hai-zhou;ZENG Hong-min;KANG Yi;WANG Xiao-ling;HUANG Shuang-zhi(Department of Pathophysiology,Zhuhai Campus of Zunyi Medical University,Guangdong Zhuhai 519041,China)

机构地区：[1]遵义医学院珠海校区病理生理学教研室,广东珠海519041

出　　处：《临床医药文献电子杂志》2017年第57期11095-11096,11098,共3页Electronic Journal of Clinical Medical Literature

基　　金：国家自然科学基金资助项目(NO.:81560133);贵州省科技合作计划项目(NO.:黔科合LH字[2015]7529号);遵义医学院博士科研启动基金

摘　　要：目的探讨6-姜酚对丙酮醛诱导的HK-2肾小管上皮细胞(HK-2细胞)损伤的保护作用。方法采用丙酮醛诱导的HK-2细胞构建细胞损伤模型,并用6-姜酚进行干预。应用CCK-8法检测HK-2细胞的存活率;DAPI染色法检测HK-2细胞凋亡形态学变化。Western blot法检测活化的caspase-3蛋白表达。结果与对照组相比,模型组细胞存活率下降,差异有统计学意义(P<0.05),并出现明显凋亡特征;而加入6-姜酚处理后,细胞存活率及活化的caspase-3蛋白水平降低,差异有统计学意义(P<0.05)。结论 6-姜酚对丙酮醛诱导的HK-2细胞损伤具有保护作用。Objective To observe the protective effect of 6-gingerol on the injury of HK-2 cells induced by methylglyoxal.Methods HK-2 cells were incubated with 400μmol/L methylglyoxal for 24h and treated with different concentrations of 6-gingerol(1,10,50,100,200μmol/L).Cell viability was measured by CCK-8 assay.The morphological changes in apoptotic cells were evaluated by DAPI.The protein level of activated caspase-3 was tested by western blot.Results Compared with control group,cell viability of HK-2 cells were significant reduced by methylglyoxal;moreover,the apoptotic cells and protein level of activated caspase-3 was increased after incubated with methylglyoxal,which was inhibited by treatment of 6-gingerol.Conclusion 6-gingerol had a protective effect on HK-2 cells against the injuries induced by methylglyoxal through raising cell viability and inhibiting apoptosis.

关 键 词：6-姜酚 丙酮醛 HK-2细胞 

分 类 号：R285.5[医药卫生—中药学]