类火星环境的低气压和水对发状念珠藻复苏的影响  

Effects of Low Gas Pressure and Water Like Mars Environment on Recovery of Nostoc flagelliforme

作　　者：褚亚东[1] 刘颖慧[1] 陆洪斌[1] 曹旭鹏[1] 薛松[1] CHU Yadong;LIU Yinghui;LU Hongbin;CAO Xupeng;XUE Song(Marine Bioengineering Group,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian116023,China)

机构地区：[1]中国科学院大连化学物理研究所海洋生物工程组,大连116023

出　　处：《载人航天》2018年第3期411-417,共7页Manned Spaceflight

基　　金：载人航天预先研究项目(010701)

摘　　要：为了考察发状念珠藻(发菜)在类火星表面半开放体系复苏的可行性,搭建了在线监测模拟类火星环境密闭体系内发菜复苏时放氧速率的试验装置,并考察了水对发菜光合放氧复苏的影响。结果显示,在气压超过5℃、15℃、25℃所对应的水饱和蒸气压时,发菜放氧最高速率分别为5、7和10μmol O_2·h^(-1)·g^(-1)(DW),分别是发菜最大放氧速率的1%、2%和3%;而在气压低于相应的水饱和蒸气压时,发菜放氧平均速率分别下降为1、2和3μmol O_2·h^(-1)·g^(-1)(DW),且随着反应时间的延长,发菜在5℃放氧速率下降为0;然而,在5℃,气压为1.7~1.9 k Pa,加入10 mL BG11时,发菜放氧速率最高上调至12μmol O_2·h^(-1)·g^(-1)(DW)。以上结果显示,水对发菜在类火星表面半开放体系复苏的影响大于气压。To investigate the recovery feasibility of Nostoc flagelliforme in a semi-open system under a Mars-like surface environment,an on-line O2 evolution rate monitoring system for N.flagelliforme recovery under a mimetic closed Mars-like surface environment was established,and the effects of water on the photosynthetic O2 evolution of N.flagelliforme was were investigated in this system.The results showed that at the temperatures of 5℃,15℃,and 25℃,when the atmospheric pressure exceeded the vapour pressures of the corresponding temperatures,the maximum O2 evolution rate of N.flagelliforme reached 5,7,and 10μmol O2·h-1·g-1(DW),respectively,which represented only 1%,2%,and 3%of the O2 evolution rate of N.flagelliforme under optimum conditions.When the atmospheric pressures were lower than the vapour pressures of the corresponding temperatures,the average O2 evolution rate of N.flagelliforme declined to 1,2,and 3μmol O2·h-1·g-1(DW),respectively.With the extension of time,the O2 evolution rate declined to zero at 5℃.However,under a temperature of 5℃and atmospheric pressure of 1.7~1.9 kPa,the addition of 10 mL BG11 medium led to an increase of maximum O2 evolution rate up to 12μmol O2·h-1·g-1(DW).These findings indicated that the effect of water on the recovery of the semi-open system un-der a Mars-like surface environment was greater than that of atmospheric pressure.

关 键 词：发菜 低气压 放氧 水 复苏 

分 类 号：Q935[生物学—微生物学]