烤烟品种大白筋599特征香气性状的物质及分子基础研究  

Study on Key Components and Molecular Basis Responsible for the Specific Aroma Traits of Flue-cured Tobacco Dabaijin599

作　　者：常爱霞[1] 屈亚芳 王国平 李洋洋 罗成刚[1] 冯全福[1] 陈志强[1] 付宪奎[1] 杨爱国[1] 胡日生 向世鹏 王元英[1] CHANG Aixia;QU Yafang;WANG Guoping;LI Yangyang;LUO Chenggang;FENG Quanfu;CHEN Zhiqiang;FU Xiankui;YANG Aiguo;HU Risheng;XIANG Shipeng;WANG Yuanying(Tobacoo Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Yuxi Zhongyan Tobacco Seed Co.,Ltd.,Yuxi,Yunnan 653100,China;Central South Agricultural Experimental Station of China Tobacco,Changsha 410004,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]玉溪中烟种子有限责任公司,云南玉溪653100 [3]中国烟草中南农业试验站,长沙410004

出　　处：《中国烟草科学》2018年第3期1-9,共9页Chinese Tobacco Science

基　　金：中国农业科学院科技创新工程(ASTIP-TRIC01);中国烟草总公司湖南省公司科技项目"烟草表面致香前体物分子模块创制及湖南主栽品种香气性状定向改良"(18-20Aa02)

摘　　要：为探索大白筋599特征香气性状的关键致香物质基础和分子机制,为特香型烤烟品种培育及工业利用奠定基础,通过不同品种(系)的表面分泌物检测及烟叶感官鉴定比较,明确大白筋599特征香气性状关键致香物质基础;通过对不同品种中调控关键致香物质合成的基因进行测序,明确大白筋599特征香气性状形成的分子基础。结果表明,烤烟品种大白筋599和香料烟品种Samsun表面分泌物中含有赖百当二萜冷杉醇,而普通烤烟G28等均不含冷杉醇。普通烤烟G28与大白筋599杂交或回交的后代品系中,含有冷杉醇的均具有大白筋599特异香气特性,不含冷杉醇的没有特异香气特性。调控冷杉醇合成的关键基因Nt CPS2的第292位碱基在不同品种间具有多态性,在大白筋599中是野生型碱基G,而在供试普通烤烟及生产主栽品种中是突变型碱基T。冷杉醇是烤烟品种大白筋599特异香气性状形成的关键致香物质。调控冷杉醇合成的关键基因Nt CPS2的第292位碱基在大白筋599及供试普通烤烟品种和主栽品种间的SNP多态性,是导致其香气特征差异的主要原因。通过遗传改良,丰富烟草表面分泌物中致香成分的种类和含量,是培育特色香气风格烤烟品种的重要途径。The objective of this study was to explore the key aroma component and molecular basis responsible for the specific aroma traits of flue-cured tobacco Dabaijin599,which will lay the foundation for developing flue-cured tobacco varieties with specific aroma traits and industrial utilization of their leaves.Leaf superficial exudates of different tobacco varieties(lines)were detected and smoking evaluation of the corresponding tobacco leaves was conducted to determine the main aroma components contributed to the specific aroma traits of Dabaijin599.Sequence polymorphism analysis of the key genes regulating the aroma component predicted was conducted to explore the molecular mechanism of specific aroma traits of Dabaijin599.The results showed that leaf superficial exudates of flue-cured tobacco Dabaijin599 and Oriental tobacco Samsun contain labdanoid diterpene abienol,while other flue-cured tobacco variety G28 etc.have no labdanoid diterpene abienol.Among the backcross or hybridization population of G28 with Dabaijin599,the offsprings contained abienol present special aroma characteristics like Dabaijin599,however,the ones without abienol had no special aroma characteristics.The 292th base of the key gene NtCPS2 controlling the synthesis of abienol was the wild type G in Dabaijin599 and the mutation type T in common flue-cured tobacco cultivars.Abienol was the key aroma component contributed to specific aroma of flue-cured tobacco variety Dabaijin599.SNP polymorphism in the 292th base of the gene NtCPS2,among Dabaijin599 and other varieties,results in the aroma characteristic difference between Dabaijin599 and the common flue-cured tobacco varieties.Enriching the type and content of aroma components by genetic improvement is an effective way to breed flue-cured tobacco varieties with special aroma.

关 键 词：大白筋599 特异香型烤烟 基因NtCPS2 冷杉醇 

分 类 号：S572.03[农业科学—烟草工业]