发酵虫草菌粉多糖成分的理化性质及其对ConA抑制L-O_2细胞增殖活性的影响  

Physicochemical Properties of Homogeneous Polysaccharide Extracted from Fermented Cordyceps Powder and It's Antagonism of Inhibition Effect on Cell Proliferation Activity of L-O_2

作　　者：蒋洪亮 麻浩 何丽芳 赵修南 单俊杰 王海南[1,3] JIANG Hong-liang;MA Hao;HE Li-fang;ZHAO Xiu-nan;SHAN Jun-jie;WANG Hai-nan(School of Pharmaceutical Sciences,Guizhou University,Guiyang 550025,China;Institute of Pharmacology and Toxicology,Institute of Military Medicine,Academy of Military Sciences,Beijing 100850,China;China Drug Administration,Beijing 100053,China)

机构地区：[1]贵州大学药学院,贵州贵阳550025 [2]军事科学院军事医学研究院毒物药物研究所,北京100850 [3]国家药品监督管理局,北京100053

出　　处：《中国现代中药》2018年第6期680-683,共4页Modern Chinese Medicine

摘　　要：目的:将发酵虫草菌粉(Cs-4)粗多糖进行分离纯化,并观察纯化产物对ConA抑制人正常肝细胞(L-O_2细胞株)增殖活性的拮抗作用。方法:发酵虫草菌粉(Cs-4)通过水提醇沉、复溶、透析、浓缩、冷冻干燥得粗多糖,采用DEAE-纤维素离子交换、sephadex-G-100柱色谱分离纯化粗多糖,MTT法检测细胞增殖活性。结果:发酵虫草菌粉(Cs-4)粗多糖经分离纯化得到均一成分,命名为CSMP-60-B-I。高效凝胶渗透色谱法测得其相对峰高分子量为32 336 Da。毛细管电泳测得单糖组成为木糖-阿拉伯糖-葡萄糖-半乳糖-甘露糖(0.09∶0.20∶1.00∶3.70∶3.58)。高浓度ConA能抑制L-O_2细胞增殖,而加入CSMP-60-B-I后,这种抑制作用大大减弱。结论:发酵虫草菌粉(Cs-4)粗多糖的分离纯化产物CSMP-60-B-I对ConA抑制L-O_2细胞增殖活性有拮抗作用,提示其可能有保肝作用。Objective:To isolate and purify the total polysaccharides extracted from fermented Cordyceps powder(Cs-4)and observe the antagonism of inhibition effect on cell proliferation activity of L-O2.Methods:The fermented Cordyceps powder(Cs-4)was extracted with water at 60℃and precipitated with ethanol,then redissolved,dialyzed,concentrated and lyophilized to obtain the total polysaccharides.The total polysaccharides were fractionated by a DEAE-cellulose column.The cell proliferation activity of L-O2 was detected by MTT.Results:The total polysaccharides extracted from fermented Cordyceps powder(Cs-4)were fractionated to obtain homogeneous polysaccharide named CSMP-60-B-I.The relative peak molecular weight of CSMP-60-B-I was 32 336 Da,determined by high performance gel permeation chromatography.The composition of monosaccharides and molar ratios were xylose-arabinose-glucose-galactose-mannose(0.09:0.20:1.00:3.70:3.58),determined by capillary electrophoresis.The results showed that ConA in high concentration could inhibit the cell proliferation activity of L-O2 in vitro.After the addition of CSMP-60-B-I,the inhibiting effect was greatly reduced.Conclusion:CSMP-60-B-I may have the effect of reducing inhibition of cell proliferation activity of L-O2by ConA in high concentration,implicating that CSMP-60-B-I might have hepatoprotective effect.

关 键 词：发酵虫草菌粉 多糖 分离纯化 肝细胞 

分 类 号：R285[医药卫生—中药学] R286.0[医药卫生—中医学]