朝鲜白头翁的HPLC特征图谱研究  

Study on HPLC Characteristic Chromatogram of Pulsatilla cernua

作　　者：任乔森 王海洋[1] 翟宏宇[1] 赵琳琳[1] 李秀芬[1] 唐秋竹[1] 赵琪钟 REN Qiao-sen;WANG Hai-yang;ZHAI Hong-yu;ZHAO Lin-lin;LI Xiu-fen;TANG Qiu-zhu;ZHAO Qi-zhong(Jilin Institute for Drug Control,Changchun 130033,China;Puer Institute for food and Drug Control,Puer 665000,China)

机构地区：[1]吉林省药品检验所,吉林长春130033 [2]普洱市食品药品检验检测中心,云南普洱665000

出　　处：《中国现代中药》2018年第6期711-715,共5页Modern Chinese Medicine

摘　　要：目的:建立朝鲜白头翁的特征图谱,为科学有效评价和控制其质量提供可靠依据。方法:采用高效液相色谱法,Thermo Hypersil BDS C_(18)色谱柱(4.6×250 mm,5μm),以乙腈-水作为流动相进行梯度洗脱,流速0.8 mL·min^(-1),柱温35℃,检测波长201 nm。结果:初步建立了朝鲜白头翁的特征图谱,标定了20个共有峰,并指认出2个。结论:采用HPLC法建立朝鲜白头翁的特征图谱,操作简单,选择性高,分离效果好,可用于朝鲜白头翁及其混淆品的鉴别和质量控制。Objective:To establish HPLC characteristic chromatograms of Pulsatilla cernua,so as to provide a reliable method for scientific evaluation and effective quality control of medicinal material.Methods:The effective components were separated by HPLC with Thermo Hypersil BDS C18(4.6×250 mm,5μm)in a gradient elution of acetonitrile and water as the mobile phase at the flow rate of 0.8 mL·min-1 and temperature of 35℃and detection wavelength of 201 nm.Results:The specific chromatogram of Pulsatilla cernua was established and 20 common peaks were indicated and 2 peaks were indicated.Conclusion:The HPLC method was precise,stable and reproducible.These established methods are practical and suitable for the identify and quality control of Pulsatilla cernua.

关 键 词：朝鲜白头翁 高效液相色谱 特征图谱 质量控制 

分 类 号：R284[医药卫生—中药学]